ArticleProceedings of the National Academy of Sciences of the United States of America2010
Energized outer membrane and spatial separation of metabolic processes in the hyperthermophilic Archaeon Ignicoccus hospitalis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 83 citations in OpenAlex.
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- Functional compartmentalization and metabolic separation in a prokaryotic cell.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Extracellular Metabolism Sets the Table for Microbial Cross-Feeding.Microbiology and molecular biology reviews : MMBR · 2021Review
- Extracellular membrane vesicles and nanotubes in Archaea.microLife · 2021Review
- Plant-archaea relationships: a potential means to improve crop production in arid and semi-arid regions.World journal of microbiology & biotechnology · 2020Review
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- Purification of a Crenarchaeal ATP Synthase in the Light of the Unique Bioenergetics ofJournal of bacteriology · 2019Article
- Spotlight on the Energy Harvest of Electroactive Microorganisms: The Impact of the Applied Anode Potential.Frontiers in microbiology · 2019Article
- Genomic diversity, lifestyles and evolutionary origins of DPANN archaea.FEMS microbiology letters · 2019Review
- Single-cell genomics of co-sorted Nanoarchaeota suggests novel putative host associations and diversification of proteins involved in symbiosis.Microbiome · 2018Article
- Article
- A Complex Endomembrane System in the ArchaeonFrontiers in microbiology · 2017Article
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5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
ATP synthase catalyzes ATP synthesis at the expense of an electrochemical ion gradient across a membrane that can be generated by different exergonic reactions. Sulfur reduction is the main energy-yielding reaction in the hyperthermophilic strictly anaerobic Crenarchaeon Ignicoccus hospitalis. This organism is unusual in having an inner and an outer membrane that are separated by a huge intermembrane compartment. Here we show, on the basis of immuno-EM analyses of ultrathin sections and immunofluorescence experiments with whole I. hospitalis cells, that the ATP synthase and H(2):sulfur oxidoreductase complexes of this organism are located in the outer membrane. These two enzyme complexes are mandatory for the generation of an electrochemical gradient and for ATP synthesis. Thus, among all prokaryotes possessing two membranes in their cell envelope (including Planctomycetes, gram-negative bacteria), I. hospitalis is a unique organism, with an energized outer membrane and ATP synthesis within the periplasmic space. In addition, DAPI staining and EM analyses showed that DNA and ribosomes are localized in the cytoplasm, leading to the conclusion that in I. hospitalis energy conservation is separated from information processing and protein biosynthesis. This raises questions regarding the function of the two membranes, the interaction between these compartments, and the general definition of a cytoplasmic membrane.
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