ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010
Rationale for the real-time and dynamic cell death assays using propidium iodide.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
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- A Simple Radioassay to Detect Nanoscale Membrane Disruption.Methods and protocols · 2023Article
- Label-Free Sensing of Cell Viability Using a Low-Cost Impedance Cytometry Device.Micromachines · 2023Article
- Thyroid hormone-induced cell death in sea urchin metamorphic development.The Journal of experimental biology · 2022Article
- Host transmission dynamics of first- and third-stageParasitology · 2022Article
- In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach.International journal of molecular sciences · 2021Review
- Integration of Engineered "Spark-Cell" Spheroids for Optical Pacing of Cardiac Tissue.Frontiers in bioengineering and biotechnology · 2021Article
- Rapid determination of nematode cell and organ susceptibility to toxic treatments.International journal for parasitology. Drugs and drug resistance · 2020Article
- An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells.Bio-protocol · 2018Article
- Article
- Reduced Intracellular Drug Accumulation in Drug-Resistant Leukemia Cells is Not Only Solely Due to MDR-Mediated Efflux but also to Decreased Uptake.Frontiers in oncology · 2014Article
- Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Review
- Standard fluorescent imaging of live cells is highly genotoxic.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Cytometry of apoptosis. Historical perspective and new advances.Experimental oncology · 2012Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 4 countries.
Funding
Abstract
We have recently reported an innovative approach to use charged fluorochromes such as propidium iodide (PI) in the real-time, dynamic cell viability assays. This study was designed to provide a mechanistic rationale for the kinetic assays using cell permeability markers. Uptake of PI by live cells, effect on the cell cycle, long-term proliferation capacity, DNA damage response, and pharmacologic interactions with anticancer drugs were studied using both laser scanning microscopy and laser scanning cytometry. Exposure of human carcinomic alveolar basal epithelial A549 cells in cultures to 1.5 or 7.5 microM of PI for 24 h had minimal effect on cell cycle progression including DNA replication as measured by incorporation of 5'-ethynyl-2-deoxyuridine (EdU) detected by the "click chemistry" approach and measured by laser scanning cytometry. A modest reduction, from 44 to 40% or 33%, in frequency of DNA replicating cells was seen after 48 h at 1.5 or 7.5 microM concentration of PI. There was no evidence of increased phosphorylation of histone gammaH2AX in cells growing in the presence of 1.5 or 7.5 microM of PI for up to 48 h. Confocal image analysis of HeLa and NIH 3T3 mouse embryonic fibroblasts growing in the presence of PI showed granular distribution in cell cytoplasm suggesting PI accumulation in endosomes and progressive increase in fluorescence of nucleoli reflecting PI binding to nucleolar RNA. The overall responses of cells to cytotoxic agents were also not affected by the growth in the presence PI. Our data lend further support to the notion that PI can be effectively used in real-time, kinetic viability assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.