Evidence map›Paper›PMID 20130277›Full record

ArticleInvestigative ophthalmology & visual science2010

Reduced connexin 43 expression and its effect on the development of vascular lesions in retinas of diabetic mice.

Michael W Bobbie, Sumon Roy, Kyle Trudeau, Stephanie J Munger, Alexander M Simon, Sayon Roy

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 105 citations in OpenAlex.

  1. Topical administration of a connexin43-based peptide augments healing of chronic neuropathic diabetic foot ulcers: A multicenter, randomized trial.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
    Trial
  2. Rediscovery of pericytes within the neurovascular unit for cerebral ischemia/reperfusion injury.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2026
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  19. Blood-Brain Barrier Mechanisms in Stroke and Trauma.Handbook of experimental pharmacology · 2022
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6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Michael W BobbieDepartments of Medicine, University of Arizona, Tucson, Arizona, USA.
Sumon Roy
Kyle Trudeau
Stephanie J Munger
Alexander M Simon
Sayon Roy
Boston University · US

Funding

Role of intercellular communication in pathogenesis of diabetic retinopathyR01EY018218 · NEI · BOSTON MEDICAL CENTER · PI ROY, SAYON · 2009 to 2021
$2.7M
Gap Junction Mediated Signaling and Vascular ResponsesR01HL064232 · NHLBI · UNIVERSITY OF ARIZONA · PI SIMON, ALEXANDER M · 2001 to 2010
$2.2M
NEI NIH HHS EY018218NEI NIH HHS R01 EY018218NHLBI NIH HHS HL064232NHLBI NIH HHS R01 HL064232
6 · The paper itself

Abstract

PURPOSE. To examine whether diabetes-induced connexin 43 downregulation promotes retinal vascular lesions characteristic of diabetic retinopathy (DR). METHODS. Two animal models, streptozotocin-induced diabetic mice and Cx43 heterozygous knockout (Cx43(+/-)) mice, were studied to directly assess whether diabetes reduces the expression of retinal Cx43, which, in turn, contributes to retinal vascular cell loss by apoptosis. Retinal Cx43 protein levels were assessed in nondiabetic control mice, diabetic mice, and Cx43(+/-) mice by Western blot analysis, and Cx43 localization and distribution in the retinal vascular cells were studied by immunostaining of retinal trypsin digests (RTDs). In parallel, RTDs were stained with hematoxylin and periodic acid Schiff to determine pericyte loss (PL) and acellular capillaries (AC), and TUNEL assays were performed to determine retinal vascular cell apoptosis. RESULTS. Western blot analysis indicated significant reductions in retinal Cx43 protein levels in diabetic mice and Cx43(+/-) mice compared with those of nondiabetic mice. Similarly, a significant reduction in Cx43 immunostaining was observed in the retinal capillaries of diabetic mice and Cx43(+/-) mice compared with those of control mice. Both diabetic and age-matched Cx43(+/-) mice exhibited increased amount of PL, AC, and TUNEL-positive cells compared with control mice. CONCLUSIONS. Diabetes-induced inhibition of Cx43 expression contributes to vascular cell apoptosis in retinas of diabetic mice. This suggests that reduced Cx43 expression plays a critical role in the development of AC and PL associated with DR.

Indexed as

Down-RegulationAnimalsApoptosisBlood GlucoseBlotting, WesternBody WeightConnexin 43Diabetes Mellitus, ExperimentalDiabetic RetinopathyGenotypeGlycemic IndexIn Situ Nick-End LabelingMiceMice, Inbred C57BLMice, KnockoutPericytesBlood GlucoseConnexin 43GJA1 protein, mouse

Identifiers

PMID20130277
PMCPMC2904019
OpenAlexW2016103347

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.