ArticleNucleic acids research2010
A novel antisense RNA regulates at transcriptional level the virulence gene icsA of Shigella flexneri.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 88 citations in OpenAlex.
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- Genome-Scale Analysis of the Structure and Function of RNA Pathways and Networks in Pseudomonas aeruginosa.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Elucidation of the DNA-Binding Activity of VirF from Shigella flexneri for theJournal of bacteriology · 2023Article
- Developing New Tools to Fight Human Pathogens: A Journey through the Advances in RNA Technologies.Microorganisms · 2022Review
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- Elucidation of Key Interactions between VirF and theJournal of bacteriology · 2022Article
- Roles of Two-Component Signal Transduction Systems in Shigella Virulence.Biomolecules · 2022Review
- The VirF21:VirF30 protein ratio is affected by temperature and impacts Shigella flexneri host cell invasion.FEMS microbiology letters · 2022Article
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- Kinetic modeling reveals additional regulation at co-transcriptional level by post-transcriptional sRNA regulators.Cell reports · 2021Article
- Topologies of synthetic gene circuit for optimal fold change activation.Nucleic acids research · 2021Article
- Diversity and Versatility in Small RNA-Mediated Regulation in Bacterial Pathogens.Frontiers in microbiology · 2021Review
- Modulation of Bacterial Fitness and Virulence Through Antisense RNAs.Frontiers in cellular and infection microbiology · 2020Review
- A Novel sRNA inFrontiers in cellular and infection microbiology · 2020Article
- Transcription ofJournal of bacteriology · 2019Article
- Defining the Transcriptional and Post-transcriptional Landscapes ofFrontiers in microbiology · 2019Article
- Molecules that Inhibit Bacterial Resistance Enzymes.Molecules (Basel, Switzerland) · 2018Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The virulence gene icsA of Shigella flexneri encodes an invasion protein crucial for host colonization by pathogenic bacteria. Within the intergenic region virA-icsA, we have discovered a new gene that encodes a non-translated antisense RNA (named RnaG), transcribed in cis on the complementary strand of icsA. In vitro transcription assays show that RnaG promotes premature termination of transcription of icsA mRNA. Transcriptional inhibition is also observed in vivo by monitoring the expression profile in Shigella by real-time polymerase chain reaction and when RnaG is provided in trans. Chemical and enzymatic probing of the leader region of icsA mRNA either free or bound to RnaG indicate that upon hetero-duplex formation an intrinsic terminator, leading to transcription block, is generated on the nascent icsA mRNA. Mutations in the hairpin structure of the proposed terminator impair the RnaG mediated-regulation of icsA transcription. This study represents the first evidence of transcriptional attenuation mechanism caused by a small RNA in Gram-negative bacteria. We also present data on the secondary structure of the antisense region of RnaG. In addition, alternatively silencing icsA and RnaG promoters, we find that transcription from the strong RnaG promoter reduces the activity of the weak convergent icsA promoter through the transcriptional interference regulation.
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