ArticlePlant molecular biology2010
Transgene excision from wheat chromosomes by phage phiC31 integrase.
Article in Plant molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 38 citations in OpenAlex.
- An integrase toolbox to record gene-expression during plant development.Nature communications · 2023Article
- Genetic switches designed for eukaryotic cells and controlled by serine integrases.Communications biology · 2020Article
- Small serine recombination systems ParA-MRS and CinH-RS2 perform precise excision of plastid DNA.Plant biotechnology journal · 2017Article
- Investigating Triticeae anther gene promoter activity in transgenic Brachypodium distachyon.Planta · 2017Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Split-gene system for hybrid wheat seed production.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The production of male-sterile wheat plants through split barnase expression is promoted by the insertion of introns and flexible peptide linkers.Transgenic research · 2013Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Article
- Transgene excision in pollen using a codon optimized serine resolvase CinH-RS2 site-specific recombination system.Plant molecular biology · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Streptomyces phage phiC31 integrase was tested for its ability to excise transgenic DNA from the wheat genome by site-specific recombination. Plants that stably express phiC31 integrase were crossed to plants carrying a target construct bearing the phiC31 recognition sites, attP and attB. In the progeny, phiC31 recombinase mediates recombination between the att sites of the target locus, which results in excision of the intervening DNA. Recombination events could be identified in 34 independent wheat lines by PCR and Southern blot analysis and by sequencing of the excision footprints. Recombinant loci were inherited to the subsequent generation. The results presented here establish the integrase-att system as a tool for catalysing the precise elimination of DNA sequences from wheat chromosomes.
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Registered trials
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