Evidence map›Paper›PMID 20127113›Full record

ArticleParasitology research2010

Molecular characterization of Blastocystis species in Oregon identifies multiple subtypes.

Christopher M Whipps, Ken Boorom, Luiz E Bermudez, Michael L Kent

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.5field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Article
  3. Identification of MultipleFrontiers in veterinary science · 2021
    Article
  4. Prevalence and Subtype Distribution ofInternational journal of environmental research and public health · 2020
    Article
  5. Article
  6. Frequency and genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Christopher M WhippsDepartment of Environmental and Forest Biology, SUNY-ESF, State University of New York College of Environmental Science and Forestry, Syracuse, NY 13210, USA. cwhipps@esf.edu
Ken Boorom
Luiz E Bermudez
Michael L Kent
Oregon State University · USSUNY College of Environmental Science and Forestry · US

Funding

WNT SIGNALING IN THE DEVELOPING GASTROINTESTINAL TRACTP20RR016463 · NCRR · MOUNT DESERT ISLAND BIOLOGICAL LAB · PI WRAY, CHRISTOPHER C · 2001 to 2011
$41.0M
NCRR NIH HHS 2 P20 RR016463
6 · The paper itself

Abstract

The association of Blastocystis species infections with gastrointestinal symptoms in humans is clouded by the variable presentation of disease and multiple lineages of the parasite that can infect humans and other animals. It has long been suspected that certain subtypes of Blastocystis may be more or less pathogenic, be restricted to certain hosts, or have limits to their geographic distribution. In the state of Oregon, USA, Blastocystis spp. are the most commonly encountered parasites in fecal specimens submitted for diagnostic evaluation, yet the diversity of subtypes is unknown. In this study, fecal samples were collected from individuals experiencing symptoms associated with blastocystosis and analyzed by polymerase chain reaction for presence of the parasite and DNA sequenced for subtyping. Five of 19 individuals tested positive for the parasite, all of which were also positive by previous ova and parasitology examination. DNA sequencing of the small subunit ribosomal DNA and elongation factor 1 alpha gene followed by phylogenetic subtyping identified five unique subtypes, representing Blastocystis subtypes 1, 2, 3, 4, and 8. No symptoms were consistently associated with presence or absence of infection, although abdominal pain and fatigue were reported by all infected individuals. Multiple subtypes are indicative of multiple sources of infection, suggesting more extensive surveys are required to understand the transmission of this parasite.

Indexed as

Genetic VariationAdultAnimalsBlastocystisBlastocystis InfectionsChildCluster AnalysisDNA, ProtozoanDNA, RibosomalDNA, Ribosomal SpacerFecesFemaleGenes, rRNAGenotypeHumansInfantDNA, ProtozoanDNA, RibosomalDNA, Ribosomal SpacerPeptide Elongation Factor 1Protozoan ProteinsRNA, ProtozoanRNA, Ribosomal, 18S

Identifiers

PMID20127113
OpenAlexW1983141185

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.