ArticlePloS one2010
Distinct activities of exonuclease 1 and flap endonuclease 1 at telomeric g4 DNA.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.Critical reviews in biochemistry and molecular biology · 2026Review
- Bacillus subtilis encodes a discrete flap endonuclease that cleaves RNA-DNA hybrids.PLoS genetics · 2023Article
- DNA Damage/Repair Management in Cancers.Cancers · 2020Review
- Formation and processing of DNA damage substrates for the hNEIL enzymes.Free radical biology & medicine · 2017Review
- Review
- RECQ1 interacts with FEN-1 and promotes binding of FEN-1 to telomeric chromatin.The Biochemical journal · 2015Article
- Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Mammalian DNA2 helicase/nuclease cleaves G-quadruplex DNA and is required for telomere integrity.The EMBO journal · 2013Article
- Flap endonuclease 1.Annual review of biochemistry · 2013Review
- Kinetics of endogenous mouse FEN1 in base excision repair.Nucleic acids research · 2012Article
- Exonuclease 1 (EXO1) gene variation and melanoma risk.DNA repair · 2012Article
- 14-3-3 checkpoint regulatory proteins interact specifically with DNA repair protein human exonuclease 1 (hEXO1) via a semi-conserved motif.DNA repair · 2012Article
- Structures of human exonuclease 1 DNA complexes suggest a unified mechanism for nuclease family.Cell · 2011Article
- Rudimentary G-quadruplex-based telomere capping in Saccharomyces cerevisiae.Nature structural & molecular biology · 2011Article
- Telomere replication: poised but puzzling.Journal of cellular and molecular medicine · 2011Review
- Telomeric repeat mutagenicity in human somatic cells is modulated by repeat orientation and G-quadruplex stability.DNA repair · 2010Article
- Complementary roles for exonuclease 1 and Flap endonuclease 1 in maintenance of triplet repeats.The Journal of biological chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundExonuclease 1 (EXO1) and Flap endonuclease 1 (FEN1) are members of the RAD2 family of structure-specific nucleases. Genetic analysis has identified roles for EXO1 and FEN1 in replication, recombination, DNA repair and maintenance of telomeres. Telomeres are composed of G-rich repeats that readily form G4 DNA. We recently showed that human EXO1 and FEN1 exhibit distinct activities on G4 DNA substrates representative of intermediates in immunoglobulin class switch recombination. METHODOLOGY/PRINCIPAL
findingsWe have now compared activities of these enzymes on telomeric substrates bearing G4 DNA, identifying non-overlapping functions that provide mechanistic insight into the distinct telomeric phenotypes caused by their deficiencies. We show that hFEN1 but not hEXO1 cleaves substrates bearing telomeric G4 DNA 5'-flaps, consistent with the requirement for FEN1 in telomeric lagging strand replication. Both hEXO1 and hFEN1 are active on substrates bearing telomeric G4 DNA tails, resembling uncapped telomeres. Notably, hEXO1 but not hFEN1 is active on transcribed telomeric G-loops. CONCLUSION/SIGNIFICANCE: Our results suggest that EXO1 may act at transcription-induced telomeric structures to promote telomere recombination while FEN1 has a dominant role in lagging strand replication at telomeres. Both enzymes can create ssDNA at uncapped telomere ends thereby contributing to recombination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.