ArticlePloS one2010
In vivo RNAi rescue in Drosophila melanogaster with genomic transgenes from Drosophila pseudoobscura.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- Parvin is essential for indirect flight muscle (IFM) performance and Zasp52 localisation to the Z-disc in Drosophila melanogaster.Cell and tissue research · 2025Article
- PSMC1 variant causes a novel neurological syndrome.Clinical genetics · 2022Article
- Tagging Drosophila Proteins with Genetically Encoded Fluorophores.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- RNA Interference (RNAi) Screening inGenetics · 2018Article
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- A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.G3 (Bethesda, Md.) · 2014Article
- RNAi screening comes of age: improved techniques and complementary approaches.Nature reviews. Molecular cell biology · 2014Review
- RNAi screening in Drosophila cells and in vivo.Methods (San Diego, Calif.) · 2014Review
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- Review
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- A role for Drosophila ATX2 in activation of PER translation and circadian behavior.Science (New York, N.Y.) · 2013Article
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- KAYAK-α modulates circadian transcriptional feedback loops in Drosophila pacemaker neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Kinesin heavy chain function in Drosophila glial cells controls neuronal activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- The genetic analysis of functional connectomics in Drosophila.Advances in genetics · 2012Review
- Article
- Extracellular inhibitors, repellents, and semaphorin/plexin/MICAL-mediated actin filament disassembly.Cytoskeleton (Hoboken, N.J.) · 2011Review
- False negative rates in Drosophila cell-based RNAi screens: a case study.BMC genomics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSystematic, large-scale RNA interference (RNAi) approaches are very valuable to systematically investigate biological processes in cell culture or in tissues of organisms such as Drosophila. A notorious pitfall of all RNAi technologies are potential false positives caused by unspecific knock-down of genes other than the intended target gene. The ultimate proof for RNAi specificity is a rescue by a construct immune to RNAi, typically originating from a related species. METHODOLOGY/PRINCIPAL
findingsWe show that primary sequence divergence in areas targeted by Drosophila melanogaster RNAi hairpins in five non-melanogaster species is sufficient to identify orthologs for 81% of the genes that are predicted to be RNAi refractory. We use clones from a genomic fosmid library of Drosophila pseudoobscura to demonstrate the rescue of RNAi phenotypes in Drosophila melanogaster muscles. Four out of five fosmid clones we tested harbour cross-species functionality for the gene assayed, and three out of the four rescue a RNAi phenotype in Drosophila melanogaster. CONCLUSIONS/SIGNIFICANCE: The Drosophila pseudoobscura fosmid library is designed for seamless cross-species transgenesis and can be readily used to demonstrate specificity of RNAi phenotypes in a systematic manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.