ArticleFrontiers in neural circuits2009
Optogenetic Dissection of Neuronal Circuits in Zebrafish using Viral Gene Transfer and the Tet System.
Article in Frontiers in neural circuits, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed, 123 citations in OpenAlex.
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- The G285S mutation in nsP1 is sufficient to render Sindbis virus as a stable vector for gene delivery.Frontiers in microbiology · 2023Article
- A new anterograde trans-synaptic tracer based on Sindbis virus.Neural regeneration research · 2022Article
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- A Confocal Microscopic Study of Gene Transfer into the Mesencephalic Tegmentum of Juvenile Chum Salmon,International journal of molecular sciences · 2021Article
- Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity.Frontiers in neuroanatomy · 2021Article
- Deep attention networks reveal the rules of collective motion in zebrafish.PLoS computational biology · 2019Article
- Regulation of forward and backward locomotion through intersegmental feedback circuits in Drosophila larvae.Nature communications · 2019Article
- Zebrafish Models of Neurodevelopmental Disorders: Limitations and Benefits of Current Tools and Techniques.International journal of molecular sciences · 2019Review
- Structural Neural Connectivity Analysis in Zebrafish With Restricted Anterograde Transneuronal Viral Labeling and Quantitative Brain Mapping.Frontiers in neural circuits · 2019Article
- Tracing of Afferent Connections in the Zebrafish Cerebellum Using Recombinant Rabies Virus.Frontiers in neural circuits · 2019Article
- Imaging of viral neuroinvasion in the zebrafish reveals that Sindbis and chikungunya viruses favour different entry routes.Disease models & mechanisms · 2017Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conditional expression of transgenes at high levels in sparse and specific populations of neurons is important for high-resolution optogenetic analyses of neuronal circuits. We explored two complementary methods, viral gene delivery and the iTet-Off system, to express transgenes in the brain of zebrafish. High-level gene expression in neurons was achieved by Sindbis and Rabies viruses. The Tet system produced strong and specific gene expression that could be modulated conveniently by doxycycline. Moreover, transgenic lines showed expression in distinct, sparse and stable populations of neurons that appeared to be subsets of the neurons targeted by the promoter driving the Tet-activator. The Tet system therefore provides the opportunity to generate libraries of diverse expression patterns similar to gene trap approaches or the thy-1 promoter in mice, but with the additional possibility to pre-select cell types of interest. In transgenic lines expressing channelrhodopsin-2, action potential firing could be precisely controlled by two-photon stimulation at low laser power, presumably because the expression levels of the Tet-controlled genes were high even in adults. In channelrhodopsin-2-expressing larvae, optical stimulation with a single blue LED evoked distinct swimming behaviors including backward swimming. These approaches provide new opportunities for the optogenetic dissection of neuronal circuit structure and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.