ArticlePloS one2010
Identification of trkH, encoding a potassium uptake protein required for Francisella tularensis systemic dissemination in mice.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- TrkA serves as a virulence modulator inFrontiers in cellular and infection microbiology · 2022Article
- The pentose phosphate pathway constitutes a major metabolic hub in pathogenic Francisella.PLoS pathogens · 2021Article
- Beyond Homeostasis: Potassium and Pathogenesis during Bacterial Infections.Infection and immunity · 2021Review
- Bactericidal fully human single-chain fragment variable antibodies protect mice against methicillin-resistantClinical & translational immunology · 2021Article
- Control ofMicroorganisms · 2020Review
- Cross Sectional Study and Risk Factors Analysis ofFrontiers in cellular and infection microbiology · 2019Article
- Two-Component Systems inFrontiers in cellular and infection microbiology · 2019Review
- Article
- Trk2 Potassium Transport System in Streptococcus mutans and Its Role in Potassium Homeostasis, Biofilm Formation, and Stress Tolerance.Journal of bacteriology · 2016Article
- The Trk Potassium Transporter Is Required for RsmB-Mediated Activation of Virulence in the Phytopathogen Pectobacterium wasabiae.Journal of bacteriology · 2016Article
- Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan.Frontiers in microbiology · 2015Article
- The contribution of the glycine cleavage system to the pathogenesis of Francisella tularensis.Microbes and infection · 2014Article
- Article
- Role for cis-acting RNA sequences in the temperature-dependent expression of the multiadhesive lig proteins in Leptospira interrogans.Journal of bacteriology · 2013Article
- The Ktr potassium transport system in Staphylococcus aureus and its role in cell physiology, antimicrobial resistance and pathogenesis.Molecular microbiology · 2013Article
- The KdpD/KdpE two-component system: integrating K⁺ homeostasis and virulence.PLoS pathogens · 2013Article
- A mariner transposon-based signature-tagged mutagenesis system for the analysis of oral infection by Listeria monocytogenes.PloS one · 2013Article
- Classical labeling of bacterial pathogens according to their lifestyle in the host: inconsistencies and alternatives.Frontiers in microbiology · 2012Article
- Article
- Francisella tularensis metabolism and its relation to virulence.Frontiers in microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Francisella tularensis is a highly infectious bacterium causing the zoonotic disease tularaemia. During its infectious cycle, F. tularensis is not only exposed to the intracellular environment of macrophages but also resides transiently in extracellular compartments, in particular during its systemic dissemination. The screening of a bank of F. tularensis LVS transposon insertion mutants on chemically defined medium (CDM) led us to identify a gene, designated trkH, encoding a homolog of the potassium uptake permease TrkH. Inactivation of trkH impaired bacterial growth in CDM. Normal growth of the mutant was only restored when CDM was supplemented with potassium at high concentration. Strikingly, although not required for intracellular survival in cell culture models, TrkH appeared to be essential for bacterial virulence in the mouse. In vivo kinetics of bacterial dissemination revealed a severe defect of multiplication of the trkH mutant in the blood of infected animals. The trkH mutant also showed impaired growth in blood ex vivo. Genome sequence analyses suggest that the Trk system constitutes the unique functional active potassium transporter in both tularensis and holarctica subspecies. Hence, the impaired survival of the trkH mutant in vivo is likely to be due to its inability to survive in the low potassium environment (1-5 mM range) of the blood. This work unravels thus the importance of potassium acquisition in the extracellular phase of the F. tularensis infectious cycle. More generally, potassium could constitute an important mineral nutrient involved in other diseases linked to systemic dissemination of bacterial pathogens.
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