ArticlePloS one2010
Agroinfiltration reduces ABA levels and suppresses Pseudomonas syringae-elicited salicylic acid production in Nicotiana tabacum.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- 'Candidatus Phytoplasma mali' SAP11-Like protein modulates expression of genes involved in energy production, photosynthesis, and defense in Nicotiana occidentalis leaves.BMC plant biology · 2024Article
- Transient proteolysis reduction ofFrontiers in plant science · 2022Article
- Monitoring Pseudomonas syringae Growth in Agroinfiltrated Leaves: The "Agromonas" Assay.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Biological Phase Separation and Biomolecular Condensates in Plants.Annual review of plant biology · 2021Review
- From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization.Environmental microbiology · 2021Article
- Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves.The Plant journal : for cell and molecular biology · 2021Article
- Engineering bacteriocin-mediated resistance against the plant pathogen Pseudomonas syringae.Plant biotechnology journal · 2020Article
- The transcriptome, extracellular proteome and active secretome of agroinfiltrated Nicotiana benthamiana uncover a large, diverse protease repertoire.Plant biotechnology journal · 2018Article
- Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato.International journal of molecular sciences · 2018Article
- Artificial Agrobacterium tumefaciens strains exhibit diverse mechanisms to repress Xanthomonas oryzae pv. oryzae-induced hypersensitive response and non-host resistance in Nicotiana benthamiana.Molecular plant pathology · 2017Article
- An Agrobacterium tumefaciens Strain with Gamma-Aminobutyric Acid Transaminase Activity Shows an Enhanced Genetic Transformation Ability in Plants.Scientific reports · 2017Article
- Unmasking host and microbial strategies in the Agrobacterium-plant defense tango.Frontiers in plant science · 2015Review
- Transcriptome Analysis of Plant Hormone-Related Tomato (Solanum lycopersicum) Genes in a Sunlight-Type Plant Factory.PloS one · 2015Article
- Agrobacterium-derived cytokinin influences plastid morphology and starch accumulation in Nicotiana benthamiana during transient assays.BMC plant biology · 2014Article
- Optimizing the transient Fast Agro-mediated Seedling Transformation (FAST) method in Catharanthus roseus seedlings.Plant cell reports · 2014Article
- Increased 1-aminocyclopropane-1-carboxylate deaminase activity enhances Agrobacterium tumefaciens-mediated gene delivery into plant cells.MicrobiologyOpen · 2013Article
- Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.Plant cell reports · 2012Article
- Cytokinins mediate resistance against Pseudomonas syringae in tobacco through increased antimicrobial phytoalexin synthesis independent of salicylic acid signaling.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundAgrobacterium tumefaciens strain GV3101 (pMP90) is widely used in transient gene expression assays, including assays to study pathogen effectors and plant disease resistance mechanisms. However, inoculation of A. tumefaciens GV3101 into Nicotiana tabacum (tobacco) leaves prior to infiltration with pathogenic and non-host strains of Pseudomonas syringae results in suppression of macroscopic symptoms when compared with leaves pre-treated with a buffer control. METHODOLOGY/
findingsTo gain further insight into the mechanistic basis of symptom suppression by A. tumefaciens we examined the effect of pre-treatment with A. tumefaciens on the growth of P. syringae, the production of the plant signalling molecules salicylic acid (SA) and abscisic acid (ABA), and the presence of callose deposits. Pre-treatment with A. tumefaciens reduced ABA levels, P. syringae multiplication and P. syringae-elicited SA and ABA production, but promoted increased callose deposition. However, pre-treatment with A. tumefaciens did not suppress necrosis or SA production in leaves inoculated with the elicitor HrpZ. CONCLUSIONS/SIGNIFICANCE: Collectively, these results show that inoculation of N. tabacum leaves with A. tumefaciens alters plant hormone levels and plant defence responses to P. syringae, and demonstrate that researchers should consider the impact of A. tumefaciens on plant signal transduction when using A. tumefaciens-mediated transient expression assays to investigate ABA-regulated processes or pathogenicity and plant defence mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.