ArticleMolecular therapy : the journal of the American Society of Gene Therapy2010
In vivo fluorescence imaging of muscle cell regeneration by transplanted EGFP-labeled myoblasts.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- A Human-derived Dual MRI/PET Reporter Gene System with High Translational Potential for Cell Tracking.Molecular imaging and biology · 2022Article
- Obestatin Increases the Regenerative Capacity of Human Myoblasts Transplanted Intramuscularly in an Immunodeficient Mouse Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Cell Therapy in Myology: Dynamics of Muscle Precursor Cell Death after Intramuscular Administration in Non-human Primates.Molecular therapy. Methods & clinical development · 2017Article
- Differentiation of Apical Bud Cells in a Newly Developed Apical Bud Transplantation Model Using GFP Transgenic Mice as Donor.PloS one · 2016Article
- Automated high-content morphological analysis of muscle fiber histology.Computers in biology and medicine · 2015Article
- An image processing approach to analyze morphological features of microscopic images of muscle fibers.Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society · 2014Article
- Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability.Stem cells (Dayton, Ohio) · 2014Article
- Options for tracking GFP-Labeled transplanted myoblasts using in vivo fluorescence imaging: implications for tracking stem cell fate.BMC biotechnology · 2014Article
- Dynamics of acute local inflammatory response after autologous transplantation of muscle-derived cells into the skeletal muscle.Mediators of inflammation · 2014Article
- Visualizing osteogenesis in vivo within a cell-scaffold construct for bone tissue engineering using two-photon microscopy.Tissue engineering. Part C, Methods · 2013Article
- Assessment of the green florescence protein labeling method for tracking implanted mesenchymal stem cells.Cytotechnology · 2012Article
- A novel micro-linear vector for in vitro and in vivo gene delivery and its application for EBV positive tumors.PloS one · 2012Article
- CD34 promotes satellite cell motility and entry into proliferation to facilitate efficient skeletal muscle regeneration.Stem cells (Dayton, Ohio) · 2011Article
- Tracking dynamics of muscle engraftment in small animals by in vivo fluorescent imaging.Journal of visualized experiments : JoVE · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In vivo fluorescence imaging (FLI) enables monitoring fluorescent protein (FP)-labeled cells and proteins in living organisms noninvasively. Here, we examined whether this modality could reach a sufficient sensitivity to allow evaluation of the regeneration process of enhanced green fluorescent protein (eGFP)-labeled muscle precursors (myoblasts). Using a basic FLI station, we were able to detect clear fluorescence signals generated by 40,000 labeled cells injected into a tibialis anterior (TA) muscle of mouse. We observed that the signal declined to approximately 25% on the 48 hours of cell injection followed by a recovery starting at the second day and reached a peak of approximately 45% of the original signal by the 7th day, suggesting that the survived population underwent a limited run of proliferation before differentiation. To assess whether transplanted myoblasts could form satellite cells, we injured the transplanted muscles repeatedly with cardiotoxin. We observed a recovery of fluorescence signal following a disappearance of the signal after each cardiotoxin injection. Histology results showed donor-derived cells located underneath basal membrane and expressing Pax7, confirming that the regeneration observed by imaging was indeed mediated by donor-derived satellite cells. Our results show that FLI is a powerful tool that can extend our ability to unveil complicated biological processes such as stem cell-mediated regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.