ArticleMolecular & cellular proteomics : MCP2010
Sub-part-per-million precursor and product mass accuracy for high-throughput proteomics on an electron transfer dissociation-enabled orbitrap mass spectrometer.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A Strategy to Achieve Sub-Parts-per-Million Mass Measurement Accuracy of N-Linked Glycans Using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization.Journal of mass spectrometry : JMS · 2025Article
- Short and long-term effects on the thyroid proteome after 131I exposure.Radiation protection dosimetry · 2025Article
- Mass recalibration for desorption electrospray ionization mass spectrometry imaging using endogenous reference ions.BMC bioinformatics · 2022Article
- Tannic Acid: a Novel Calibrator for Facile and Accurate Mass Measurement of Electrospray Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- Neucode Labels for Multiplexed, Absolute Protein Quantification.Analytical chemistry · 2016Article
- High-resolution filtering for improved small molecule identification via GC/MS.Analytical chemistry · 2015Article
- Automated deconvolution of overlapped ion mobility profiles.Journal of the American Society for Mass Spectrometry · 2014Article
- Mass accuracy and isotopic abundance measurements for HR-MS instrumentation: capabilities for non-targeted analyses.Journal of the American Society for Mass Spectrometry · 2014Article
- Induced dual-nanospray: a novel internal calibration method for convenient and accurate mass measurement.Journal of the American Society for Mass Spectrometry · 2013Article
- Mass recalibration of FT-ICR mass spectrometry imaging data using the average frequency shift of ambient ions.Journal of the American Society for Mass Spectrometry · 2013Article
- A proteomics search algorithm specifically designed for high-resolution tandem mass spectra.Journal of proteome research · 2013Article
- Gas-phase ion/ion reactions of peptides and proteins: acid/base, redox, and covalent chemistries.Chemical communications (Cambridge, England) · 2013Article
- Advanced mass calibration and visualization for FT-ICR mass spectrometry imaging.Journal of the American Society for Mass Spectrometry · 2012Article
- Instant spectral assignment for advanced decision tree-driven mass spectrometry.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis.Molecular & cellular proteomics : MCP · 2011Article
- Approaches for the study of cancer: towards the integration of genomics, proteomics and metabolomics.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2011Review
- Characterization and diagnostic value of amino acid side chain neutral losses following electron-transfer dissociation.Journal of the American Society for Mass Spectrometry · 2011Article
- Mass spectrometry in chronic kidney disease research.Advances in chronic kidney disease · 2010Review
Corrections and comments
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Authors and funding
4 authors.
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Abstract
We demonstrate a new approach for internal mass calibration on an electron transfer dissociation-enabled linear ion trap-orbitrap hybrid mass spectrometer. Fluoranthene cations, a byproduct of the reaction used for generation of electron transfer dissociation reagent anions, are co-injected with the analyte cations in all orbitrap mass analysis events. The fluoranthene cations serve as a robust internal calibrant with minimal impact on scan time (<20 ms) or spectral quality. Following external mass calibration, 60 replicate LC-MS/MS runs of a complex peptide mixture were collected over the course of approximately 136 h (almost 6 days). Using only standard external mass calibration, the mass accuracy for a typical analysis was -3.31 +/- 0.93 ppm (sigma) for precursors and -2.32 +/- 0.89 ppm for products. After application of internal recalibration, mass accuracy improved to +0.77 +/- 0.71 ppm for precursors and +0.17 +/- 0.67 ppm for products. When all 60 replicate runs were analyzed together without internal mass recalibration, the mass accuracy was -1.23 +/- 1.54 ppm for precursors and -0.18 +/- 1.42 ppm for products, nearly a 2-fold drop in precision relative to an individual run. After internal mass recalibration, this improved to +0.80 +/- 0.70 ppm for precursors and +0.16 +/- 0.67 ppm for products, roughly equivalent to that obtained in a single run, demonstrating a near complete elimination of mass calibration drift.
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Registered trials
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