ArticleGenetics2010
Multiple functional domains of the yeast l,3-beta-glucan synthase subunit Fks1p revealed by quantitative phenotypic analysis of temperature-sensitive mutants.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Proteomic Analysis Revealed that DVL, a Lectin Purified fromACS omega · 2025Article
- OsDISMO1: A Novel Transporter for Molybdenum Distribution in Rice Shoots.Rice (New York, N.Y.) · 2025Article
- Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.iScience · 2024Article
- Application of unimodal probability distribution models for morphological phenotyping of budding yeast.FEMS yeast research · 2024Article
- Jerveratrum-Type Steroidal Alkaloids Inhibit β-1,6-Glucan Biosynthesis in Fungal Cell Walls.Microbiology spectrum · 2022Article
- Genome-Wide Mutant Screening in Yeast Reveals that the Cell Wall is a First Shield to Discriminate Light From Heavy Lanthanides.Frontiers in microbiology · 2022Article
- Elevated Vacuolar Uptake of Fluorescently Labeled Antifungal Drug Caspofungin Predicts Echinocandin Resistance in Pathogenic Yeast.ACS central science · 2020Article
- CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew.BMC plant biology · 2020Article
- Callose Synthesis Suppresses Cell Death Induced by Low-Calcium Conditions in Leaves.Plant physiology · 2020Article
- Length Specificity and Polymerization Mechanism of (1,3)-β-d-Glucan Synthase in Fungal Cell Wall Biosynthesis.Biochemistry · 2020Article
- Genome Survey and Transcriptome Analysis on Mycelia and Primordia ofBioMed research international · 2020Article
- Genome sequence of the cauliflower mushroom Sparassis crispa (Hanabiratake) and its association with beneficial usage.Scientific reports · 2018Article
- Reduced vacuolar β-1,3-glucan synthesis affects carbohydrate metabolism as well as plastid homeostasis and structure inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Global study of holistic morphological effectors in the budding yeast Saccharomyces cerevisiae.BMC genomics · 2018Article
- The Late S-Phase Transcription Factor Hcm1 Is Regulated through Phosphorylation by the Cell Wall Integrity Checkpoint.Molecular and cellular biology · 2016Article
- Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants.Plant signaling & behavior · 2016Review
- Single-cell phenomics in budding yeast.Molecular biology of the cell · 2015Review
- Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.Molecular biology of the cell · 2015Article
- Cloning and Molecular Characterization of β-1,3-Glucan Synthase from Sparassis crispa.Mycobiology · 2014Article
- Distinct roles of cell wall biogenesis in yeast morphogenesis as revealed by multivariate analysis of high-dimensional morphometric data.Molecular biology of the cell · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main filamentous structural component of the cell wall of the yeast Saccharomyces cerevisiae is 1,3-beta-glucan, which is synthesized by a plasma membrane-localized enzyme called 1,3-beta-glucan synthase (GS). Here we analyzed the quantitative cell morphology and biochemical properties of 10 different temperature-sensitive mutants of FKS1, a putative catalytic subunit of GS. To untangle their pleiotropic phenotypes, the mutants were classified into three functional groups. In the first group, mutants fail to synthesize 1,3-beta-glucan at the proper subcellular location, although GS activity is normal in vitro. In the second group, mutants have normal 1,3-beta-glucan content but are defective in polarized growth and endocytosis. In the third group, mutations in the putative catalytic domain of Fks1p result in a loss of the catalytic activity of GS. The differences among the three groups suggest that Fks1p consists of multiple domains that are required for cell wall construction and cellular morphogenesis.
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