ArticleMolecular and cellular biology2010
Multimodal regulation of E2F1 gene expression by progestins.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Androgen receptor monomers and dimers regulate opposing biological processes in prostate cancer cells.Nature communications · 2024Article
- GATA2 and Progesterone Receptor Interaction in Endometrial Stromal Cells Undergoing Decidualization.Endocrinology · 2020Article
- Synergistic Activation of Bovine Herpesvirus 1 Productive Infection and Viral Regulatory Promoters by the Progesterone Receptor and Krüppel-Like Transcription Factor 15.Journal of virology · 2019Article
- KLF15 Inhibits Cell Proliferation in Gastric Cancer Cells via Up-Regulating CDKN1A/p21 and CDKN1C/p57 Expression.Digestive diseases and sciences · 2017Article
- Progesterone receptor blockade in human breast cancer cells decreases cell cycle progression through G2/M by repressing G2/M genes.BMC cancer · 2016Article
- Uterine ALK3 is essential during the window of implantation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- KLF15 in breast cancer: a novel tumor suppressor?Cellular oncology (Dordrecht, Netherlands) · 2015Article
- Progesterone receptor transcriptome and cistrome in decidualized human endometrial stromal cells.Endocrinology · 2015Article
- Krüppel-like factors are effectors of nuclear receptor signaling.General and comparative endocrinology · 2014Review
- Pregnancy without progesterone in horses defines a second endogenous biopotent progesterone receptor agonist, 5α-dihydroprogesterone.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The glucocorticoid receptor and KLF15 regulate gene expression dynamics and integrate signals through feed-forward circuitry.Molecular and cellular biology · 2013Article
- The requirement for p42/p44 MAPK activity in progesterone receptor-mediated gene regulation is target gene-specific.Steroids · 2013Article
- Treatment for Uterine Fibroids: Searching for Effective Drug Therapies.Drug discovery today. Therapeutic strategies · 2012Article
- Article
- Bioinformatic detection of E47, E2F1 and SREBP1 transcription factors as potential regulators of genes associated to acquisition of endometrial receptivity.Reproductive biology and endocrinology : RB&E · 2011Article
- Mechanisms of progesterone receptor inhibition of inflammatory responses in cellular models of breast cancer.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
An analysis of mRNA expression in T47D breast cancer cells treated with the synthetic progestin R5020 revealed a subset of progesterone receptor (PR) target genes that are enriched for E2F binding sites. Following up on this observation, we determined that PR-B acts in both direct and indirect manners to positively upregulate E2F1 expression in T47D cells. The direct effects of PR on E2F1 expression were confirmed by chromatin immunoprecipitation (ChIP) analysis, which indicated that the agonist-bound receptor was recruited to several enhancer elements proximal to the E2F1 transcript. However, we also noted that cycloheximide partially inhibits R5020 induction of E2F1 expression, indicating that the ligand-dependent actions of PR on this gene may involve additional indirect regulatory pathways. In support of this hypothesis, we demonstrated that treatment with R5020 significantly increases both hyperphosphorylation of Rb and recruitment of E2F1 to its own promoter, thus activating a positive feedback loop that further amplifies its transcription. Furthermore, we established that PR-mediated induction of Krüppel-like factor 15 (KLF15), which can bind to GC-rich DNA within the E2F1 promoter, is required for maximal induction of E2F1 expression by progestins. Taken together, these results suggest a new paradigm for multimodal regulation of target gene expression by PR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.