ArticleNucleic acids research2010
Preparation and topology of the Mediator middle module.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- MC EMiNEM maps the interaction landscape of the Mediator.PLoS computational biology · 2012Pooled it
- PsAvh109 suppresses SA-triggered immunity by mimicking TPL function to disrupt mediator complex assembly.Nature communications · 2026Article
- NLRs derepress MED10b- and MED7-mediated repression of jasmonate-dependent transcription to activate immunity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Interaction map of Arabidopsis Mediator complex expounding its topology.Nucleic acids research · 2019Article
- Role of integrative structural biology in understanding transcriptional initiation.Methods (San Diego, Calif.) · 2019Review
- Twenty years of Mediator complex structural studies.Biochemical Society transactions · 2019Review
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- The diverse and expanding role of mass spectrometry in structural and molecular biology.The EMBO journal · 2016Review
- Evidence for Multiple Mediator Complexes in Yeast Independently Recruited by Activated Heat Shock Factor.Molecular and cellular biology · 2016Article
- Evolution of disorder in Mediator complex and its functional relevance.Nucleic acids research · 2016Article
- Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo.Nucleic acids research · 2015Article
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- Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.Cell · 2014Article
- Model of the Mediator middle module based on protein cross-linking.Nucleic acids research · 2013Article
- Epigenetic-induced repression of microRNA-205 is associated with MED1 activation and a poorer prognosis in localized prostate cancer.Oncogene · 2013Article
- Native tandem and ion mobility mass spectrometry highlight structural and modular similarities in clustered-regularly-interspaced shot-palindromic-repeats (CRISPR)-associated protein complexes from Escherichia coli and Pseudomonas aeruginosa.Molecular & cellular proteomics : MCP · 2012Article
- Joining forces: integrating proteomics and cross-linking with the mass spectrometry of intact complexes.Molecular & cellular proteomics : MCP · 2012Review
- The Mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator is the central coactivator complex required for regulated transcription by RNA polymerase (Pol) II. Mediator consists of 25 subunits arranged in the head, middle, tail and kinase modules. Structural and functional studies of Mediator are limited by the availability of protocols for the preparation of recombinant modules. Here, we describe protocols for obtaining pure endogenous and recombinant complete Mediator middle module from Saccharomyces cerevisiae that consists of seven subunits: Med1, 4, 7, 9, 10, 21 and 31. Native mass spectrometry reveals that all subunits are present in equimolar stoichiometry. Ion-mobility mass spectrometry, limited proteolysis, light scattering and small-angle X-ray scattering all indicate a high degree of intrinsic flexibility and an elongated shape of the middle module. Protein-protein interaction assays combined with previously published data suggest that the Med7 and Med4 subunits serve as a binding platform to form the three heterodimeric subcomplexes, Med7N/21, Med7C/31 and Med4/9. The subunits, Med1 and Med10, which bridge to the Mediator tail module, bind to both Med7 and Med4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.