ArticleInfection and immunity2010
GroEL and lipopolysaccharide from Francisella tularensis live vaccine strain synergistically activate human macrophages.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Identification of theFrontiers in cellular and infection microbiology · 2025Article
- Shotgun Immunoproteomic Approach for the Discovery of Linear B-Cell Epitopes in Biothreat AgentsFrontiers in immunology · 2021Article
- SON DNA-binding protein mediates macrophage autophagy and responses to intracellular infection.FEBS letters · 2020Article
- Genomic Characterization of Antimicrobial Resistance, Virulence, and Phylogeny of the GenusAntibiotics (Basel, Switzerland) · 2020Article
- Contributions of TolC Orthologs toInfection and immunity · 2019Article
- Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins FromFrontiers in immunology · 2019Article
- Phasevarion-Regulated Virulence in the Emerging Pediatric Pathogen Kingella kingae.Infection and immunity · 2017Article
- Characterization and Vaccine Potential of Membrane Vesicles Produced by Francisella noatunensis subsp. orientalis in an Adult Zebrafish Model.Clinical and vaccine immunology : CVI · 2017Article
- Development of a Multivalent Subunit Vaccine against Tularemia Using Tobacco Mosaic Virus (TMV) Based Delivery System.PloS one · 2015Article
- B-cell epitopes in GroEL of Francisella tularensis.PloS one · 2014Article
- FeoB-mediated uptake of iron by Francisella tularensis.Infection and immunity · 2013Article
- Production of outer membrane vesicles and outer membrane tubes by Francisella novicida.Journal of bacteriology · 2013Article
- Genome sequence of Francisella tularensis subspecies holarctica strain FSC200, isolated from a child with tularemia.Journal of bacteriology · 2012Article
- Francisella tularensis reveals a disparity between human and mouse NLRP3 inflammasome activation.The Journal of biological chemistry · 2011Article
- Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins are virulence determinants in infectious disease.Infection and immunity · 2011Review
- Identification of T-cell epitopes in Francisella tularensis using an ordered protein array of serological targets.Immunology · 2011Article
- TLR4-dependent activation of inflammatory cytokine response in macrophages by Francisella elongation factor Tu.Cellular immunology · 2011Article
- Article
- The francisella tularensis proteome and its recognition by antibodies.Frontiers in microbiology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Francisella tularensis, the causative agent of tularemia, interacts with host cells of innate immunity in an atypical manner. For most Gram-negative bacteria, the release of lipopolysaccharide (LPS) from their outer membranes stimulates an inflammatory response. When LPS from the attenuated live vaccine strain (LVS) or the highly virulent Schu S4 strain of F. tularensis was incubated with human umbilical vein endothelial cells, neither species of LPS induced expression of the adhesion molecule E-selectin or secretion of the chemokine CCL2. Moreover, a high concentration (10 microg/ml) of LVS or Schu S4 LPS was required to stimulate production of CCL2 by human monocyte-derived macrophages (huMDM). A screen for alternative proinflammatory factors of F. tularensis LVS identified the heat shock protein GroEL as a potential candidate. Recombinant LVS GroEL at a concentration of 10 microg/ml elicited secretion of CXCL8 and CCL2 by huMDM through a TLR4-dependent mechanism. When 1 microg of LVS GroEL/ml was added to an equivalent amount of LVS LPS, the two components synergistically activated the huMDM to produce CXCL8. Schu S4 GroEL was less stimulatory than LVS GroEL and showed a lesser degree of synergy when combined with Schu S4 LPS. These findings suggest that the intrinsically low proinflammatory activity of F. tularensis LPS may be increased in the infected human host through interactions with other components of the bacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.