ArticleInfection and immunity2010
Critical role of dispensable genes in Mycoplasma agalactiae interaction with mammalian cells.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 42 citations in OpenAlex.
- Nucleoside binding by a surface lipoprotein governs conjugative ICE acquisition in mycoplasmas.mBio · 2026Article
- Regulation of potassium homeostasis inFrontiers in microbiology · 2026Article
- Interaction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.Mobile DNA · 2025Article
- Bacterial conjugation in the ruminant pathogenApplied and environmental microbiology · 2025Article
- Novel Nuclease MbovP701 with a Yqaj Domain Is Interrelated with the Growth ofMicroorganisms · 2024Article
- Mycoplasma bovis 5'-nucleotidase is a virulence factor conferring mammary fitness in bovine mastitis.PLoS pathogens · 2024Article
- Genes required for survival and proliferation ofApplied and environmental microbiology · 2024Article
- The Activation of the RIG-I/MDA5 Signaling Pathway upon Influenza D Virus Infection Impairs the Pulmonary Proinflammatory Response Triggered by Mycoplasma bovis Superinfection.Journal of virology · 2023Article
- Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation.Frontiers in microbiology · 2023Review
- Impacts ofMicrobial genomics · 2022Article
- Bacterial Conjugation Protocol for Ruminant Mycoplasmas.Bio-protocol · 2021Article
- Metal utilization in genome-reduced bacteria: Do human mycoplasmas rely on iron?Computational and structural biotechnology journal · 2021Review
- Novel Secreted Protein ofFrontiers in immunology · 2021Article
- An emerging role for cyclic dinucleotide phosphodiesterase and nanoRNase activities in Mycoplasma bovis: Securing survival in cell culture.PLoS pathogens · 2020Article
- Mbov_0503 Encodes a Novel Cytoadhesin that FacilitatesMicroorganisms · 2020Article
- Extracellular DNA: A Nutritional Trigger ofFrontiers in microbiology · 2019Article
- Large-Scale Analysis of theFrontiers in microbiology · 2019Article
- Mycoplasma Chromosomal Transfer: A Distributive, Conjugative Process Creating an Infinite Variety of Mosaic Genomes.Frontiers in microbiology · 2019Article
- The Integrative Conjugative Element (ICE) ofmBio · 2018Article
- Genetic loci of Mycoplasma agalactiae involved in systemic spreading during experimental intramammary infection of sheep.Veterinary research · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasmas are minimal bacteria whose genomes barely exceed the smallest amount of information required to sustain autonomous life. Despite this apparent simplicity, several mycoplasmas are successful pathogens of humans and animals, in which they establish intimate interactions with epithelial cells at mucosal surfaces. To identify biological functions mediating mycoplasma interactions with mammalian cells, we produced a library of transposon knockout mutants in the ruminant pathogen Mycoplasma agalactiae and used this library to identify mutants displaying a growth-deficient pheonotype in cell culture. M. agalactiae mutants displaying a 3-fold reduction in CFU titers to nearly complete extinction in coculture with HeLa cells were identified. Mapping of transposon insertion sites revealed 18 genomic regions putatively involved in the interaction of M. agalactiae with HeLa cells. Several of these regions encode proteins with features of membrane lipoproteins and/or were involved in horizontal gene transfer with phylogenetically distant pathogenic mycoplasmas of ruminants. Two mutants with the most extreme phenotype carry a transposon in a genomic region designated the NIF locus which encodes homologues of SufS and SufU, two proteins presumably involved in [Fe-S] cluster biosynthesis in Gram-positive bacteria. Complementation studies confirmed the conditional essentiality of the NIF locus, which was found to be critical for proliferation in the presence of HeLa cells and several other mammalian cell lines but dispensable for axenic growth. While our results raised questions regarding essential functions in mycoplasmas, they also provide a means for studying the role of mycoplasmas as minimal pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.