ArticleTissue engineering. Part C, Methods2010
Rat parotid gland cell differentiation in three-dimensional culture.
Article in Tissue engineering. Part C, Methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Review
- Slow hydrogel matrix degradation enhances salivary gland mimetic phenotype.Acta biomaterialia · 2023Article
- A combination treatment of low-dose dexamethasone and aspirin-triggered resolvin D1 reduces Sjögren syndrome-like features in a mouse model.JADA foundational science · 2023Article
- Hydrogels for Salivary Gland Tissue Engineering.Gels (Basel, Switzerland) · 2022Review
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Current experimental methods to investigate the impact of specialized pro-resolving lipid mediators on Sjögren's syndrome.Frontiers in immunology · 2022Review
- Trimers Conjugated to Fibrin Hydrogels Promote Salivary Gland Function.Journal of dental research · 2021Article
- Cell adhesion molecules' altered profile in benign and malignant salivary gland tumors. The paradigm of beta4-integrin, desmoglein-2, ICAM-1 and CD44s.Journal of biological research (Thessalonike, Greece) · 2020Article
- Engineering the mode of morphogenetic signal presentation to promote branching from salivary gland spheroids in 3D hydrogels.Acta biomaterialia · 2020Article
- Strategies for Developing Functional Secretory Epithelia from Porcine Salivary Gland Explant Outgrowth Culture Models.Biomolecules · 2019Article
- Aspirin Triggered Resolvin D1 reduces inflammation and restores saliva secretion in a Sjögren's syndrome mouse model.Rheumatology (Oxford, England) · 2019Article
- Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated αFrontiers in cell and developmental biology · 2019Article
- Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment.American journal of physiology. Regulatory, integrative and comparative physiology · 2018Article
- FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids.Journal of cell science · 2018Article
- Functional characterization and genomic studies of a novel murine submandibular gland epithelial cell line.PloS one · 2018Article
- Elastin-PLGA hybrid electrospun nanofiber scaffolds for salivary epithelial cell self-organization and polarization.Acta biomaterialia · 2017Article
- Laminin-111 Peptides Conjugated to Fibrin Hydrogels Promote Formation of Lumen Containing Parotid Gland Cell Clusters.Biomacromolecules · 2016Article
- Neurons Self-Organize Around Salivary Epithelial Cells in Novel Co-Culture Model.Journal of stem cell and regenerative biology · 2016Article
- Stem Cell-Soluble Signals Enhance Multilumen Formation in SMG Cell Clusters.Journal of dental research · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The use of polarized salivary gland cell monolayers has contributed to our understanding of salivary gland physiology. However, these cell models are not representative of glandular epithelium in vivo, and, therefore, are not ideal for investigating salivary epithelial functions. The current study has developed a three-dimensional (3D) cell culture model for rat Par-C10 parotid gland cells that forms differentiated acinar-like spheres on Matrigel. These 3D Par-C10 acinar-like spheres display characteristics similar to differentiated acini in salivary glands, including cell polarization, tight junction (TJ) formation required to maintain transepithelial potential difference, basolateral expression of aquaporin-3 and Na+/K+/2Cl- cotransporter-1, and responsiveness to the muscarinic receptor agonist carbachol that is decreased by the anion channel blocker diphenylamine-2-carboxylic acid or chloride replacement with gluconate. Incubation of the spheres in the hypertonic medium increased the expression level of the water channel aquaporin-5. Further, the proinflammatory cytokines tumor necrosis factor-alpha and interferon-gamma induced alterations in TJ integrity in the acinar-like spheres without affecting individual cell viability, suggesting that cytokines may affect salivary gland function by altering TJ integrity. Thus, 3D Par-C10 acinar-like spheres represent a novel in vitro model to study physiological and pathophysiological functions of differentiated acini.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.