ArticleApplied and environmental microbiology2010
Detection and quantification of infectious avian influenza A (H5N1) virus in environmental water by using real-time reverse transcription-PCR.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Human and Hunting Dog Interactions in the United States: Exploring Potential Transmission Pathways of Zoonotic Diseases and Highly Pathogenic Avian Influenza Virus.Veterinary sciences · 2026Article
- Genomic-based biosurveillance for avian influenza: whole genome sequencing from wild mallards sampled during autumn migration in 2022-2023 reveals a high co-infection rate on migration stopover site in Georgia.Frontiers in microbiology · 2026Article
- Risk of infection of dairy cattle in the EU with highly pathogenic avian influenza virus affecting dairy cows in the United States of America (H5N1, Eurasian lineage goose/Guangdong clade 2.3.4.4b. genotype B3.13).EFSA journal. European Food Safety Authority · 2025Article
- Detection of Hemagglutinin H5 Influenza A Virus RNA and Model of Potential Inputs in an Urban California Sewershed.Environmental science & technology · 2025Article
- Development of a Large-Volume Concentration Method to Recover Infectious Avian Influenza Virus from the Aquatic Environment.Viruses · 2024Article
- The comparison of decay rates of infectious SARS-CoV-2 and viral RNA in environmental waters and wastewater.The Science of the total environment · 2024Article
- Persistence of human respiratory viral RNA in wastewater-settled solids.Applied and environmental microbiology · 2024Article
- A sustainable approach for the removal methods and analytical determination methods of antiviral drugs from water/wastewater: A review.Journal of water process engineering · 2022Review
- Severe acute respiratory syndrome coronavirus 2 and influenza A virus co-infection alters viral tropism and haematological composition in Syrian hamsters.Transboundary and emerging diseases · 2022Article
- Surveillance of the spread of avian influenza virus type A in live bird markets in Tripoli, Libya, and determination of the associated risk factors.Veterinary world · 2022Article
- Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses.Archives of virology · 2022Article
- Comparative pathogenicity and environmental transmission of recent highly pathogenic avian influenza H5 viruses.Emerging microbes & infections · 2021Article
- RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference.Molecular therapy. Nucleic acids · 2020Article
- Detection of Low Pathogenic Avian Influenza Virus Subtype H10N7 in Poultry and Environmental Water Samples During a Clinical Outbreak in Commercial Free-Range Layers, Netherlands 2017.Frontiers in veterinary science · 2020Article
- Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides.Scientific reports · 2019Article
- Risk Factors for Avian Influenza H9 Infection of Chickens in Live Bird Retail Stalls of Lahore District, Pakistan 2009-2010.Scientific reports · 2018Article
- Cathepsin B plays a key role in optimal production of the influenza A virus.Journal of virology & antiviral research · 2018Article
- Influenza virus segment 5 (+)RNA - secondary structure and new targets for antiviral strategies.Scientific reports · 2017Article
- Risk factors for avian influenza virus contamination of live poultry markets in Zhejiang, China during the 2015-2016 human influenza season.Scientific reports · 2017Article
- A sensitive haemadsorption technique based RT-PCR for concentration and detection of Newcastle disease virus from clinical samples and allantoic fluid.Virusdisease · 2016Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Routes of avian influenza virus (AIV) dispersal among aquatic birds involve direct (bird-to-bird) and indirect (waterborne) transmission. The environmental persistence of H5N1 virus in natural water reservoirs can be assessed by isolation of virus in embryonated chicken eggs. Here we describe development and evaluation of a real-time quantitative reverse transcription (RT)-PCR (qRT-PCR) method for detection of H5N1 AIV in environmental water. This method is based on adsorption of virus particles to formalin-fixed erythrocytes, followed by qRT-PCR detection. The numbers of hemagglutinin RNA copies from H5N1 highly pathogenic AIV particles adsorbed to erythrocytes detected correlated highly with the infectious doses of the virus that were determined for three different types of artificially inoculated environmental water over a 17-day incubation period. The advantages of this method include detection and quantification of infectious H5N1 AIVs with a high level of sensitivity, a wide dynamic range, and reproducibility, as well as increased biosecurity. The lowest concentration of H5N1 virus that could be reproducibly detected was 0.91 50% egg infective dose per ml. In addition, a virus with high virion stability (Tobacco mosaic virus) was used as an internal control to accurately monitor the efficiency of RNA purification, cDNA synthesis, and PCR amplification for each individual sample. This detection system could be useful for rapid high-throughput monitoring for the presence of H5N1 AIVs in environmental water and in studies designed to explore the viability and epidemiology of these viruses in different waterfowl ecosystems. The proposed method may also be adapted for detection of other AIVs and for assessment of their prevalence and distribution in environmental reservoirs.
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