ArticleApplied and environmental microbiology2010
Genes involved in Cronobacter sakazakii biofilm formation.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 104 citations in OpenAlex.
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- Efficient inhibition of Cronobacter biofilms by chitooligosaccharides of specific molecular weight.World journal of microbiology & biotechnology · 2019Article
- Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in FoodborneFrontiers in microbiology · 2019Article
- New virulence factor CSK29544_02616 as LpxA binding partner in Cronobacter sakazakii.Scientific reports · 2018Article
- The Glutaredoxin Gene,Frontiers in microbiology · 2018Article
- Inhibition of Cronobacter sakazakii Virulence Factors by Citral.Scientific reports · 2017Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cronobacter spp. are opportunistic food-borne pathogens that can cause severe and sometimes lethal infections in neonates. In some outbreaks, the sources of infection were traced to contaminated powdered infant formula (PIF) or contaminated utensils used for PIF reconstitution. In this study, we investigated biofilm formation in Cronobacter sakazakii strain ES5. To investigate the genetic basis of biofilm formation in Cronobacter on abiotic surfaces, we screened a library of random transposon mutants of strain ES5 for reduced biofilm formation using a polystyrene microtiter assay. Genetic characterization of the mutants led to identification of genes that are associated with cellulose biosynthesis and flagellar structure and biosynthesis and genes involved in basic cellular processes and virulence, as well as several genes whose functions are currently unknown. In two of the mutants, hypothetical proteins ESA_00281 and ESA_00282 had a strong impact on flow cell biofilm architecture, and their contribution to biofilm formation was confirmed by genetic complementation. In addition, adhesion of selected biofilm formation mutants to Caco-2 intestinal epithelial cells was investigated. Our findings suggest that flagella and hypothetical proteins ESA_00281 and ESA_00282, but not cellulose, contribute to adhesion of Cronobacter to this biotic surface.
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