ArticlePlant physiology2010
Continuous turnover of carotenes and chlorophyll a in mature leaves of Arabidopsis revealed by 14CO2 pulse-chase labeling.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 148 citations in OpenAlex.
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- Divergent contribution of the MVA and MEP pathways to the formation of polyprenols and dolichols in Arabidopsis.The Biochemical journal · 2023Article
- Review of Lipid Biomarkers and Signals of Photooxidative Stress in Plants.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Combination of long-termPlant methods · 2022Article
- Functional characterization and comparison of lycopene epsilon-cyclase genes in Nicotiana tabacum.BMC plant biology · 2022Article
- Triplet-driven chemical reactivity of β-carotene and its biological implications.Nature communications · 2022Article
- The terminal enzymes of (bacterio)chlorophyll biosynthesis.Royal Society open science · 2022Review
- Color recycling: metabolization of apocarotenoid degradation products suggests carbon regeneration via primary metabolic pathways.Plant cell reports · 2022Article
- A foliar pigment-based bioassay for interrogating chloroplast signalling revealed that carotenoid isomerisation regulates chlorophyll abundance.Plant methods · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Mutation in Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase GeneFrontiers in plant science · 2022Article
- Inhibition of antioxidant enzyme activities enhances carotenogenesis in microalgaFrontiers in bioengineering and biotechnology · 2022Article
- Review
- Combined Acute Ozone and Water Stress Alters the Quantitative Relationships between OMolecules (Basel, Switzerland) · 2021Article
- Factors Differentiating the Antioxidant Activity of Macular Xanthophylls in the Human Eye Retina.Antioxidants (Basel, Switzerland) · 2021Review
- Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.Plant methods · 2021Article
- Plant apocarotenoid metabolism utilizes defense mechanisms against reactive carbonyl species and xenobiotics.Plant physiology · 2021Article
- Too rigid to fold: Carotenoid-dependent decrease in thylakoid fluidity hampers the formation of chloroplast grana.Plant physiology · 2021Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoid turnover was investigated in mature leaves of Arabidopsis (Arabidopsis thaliana) by 14CO2 pulse-chase labeling under control-light (CL; 130 micromol photons m(-2) s(-1)) and high-light (HL; 1,000 micromol photons m(-2) s(-1)) conditions. Following a 30-min 14CO2 administration, photosynthetically fixed 14C was quickly incorporated in beta-carotene (beta-C) and chlorophyll a (Chl a) in all samples during a chase of up to 10 h. In contrast, 14C was not detected in Chl b and xanthophylls, even when steady-state amounts of the xanthophyll-cycle pigments and lutein increased markedly, presumably by de novo synthesis, in CL-grown plants under HL. Different light conditions during the chase did not affect the 14C fractions incorporated in beta-C and Chl a, whereas long-term HL acclimation significantly enhanced 14C labeling of Chl a but not beta-C. Consequently, the maximal 14C signal ratio between beta-C and Chl a was much lower in HL-grown plants (1:10) than in CL-grown plants (1:4). In lut5 mutants, containing alpha-carotene (alpha-C) together with reduced amounts of beta-C, remarkably high 14C labeling was found for alpha-C while the labeling efficiency of Chl a was similar to that of wild-type plants. The maximum 14C ratios between carotenes and Chl a were 1:2 for alpha-C:Chl a and 1:5 for beta-C:Chl a in CL-grown lut5 plants, suggesting high turnover of alpha-C. The data demonstrate continuous synthesis and degradation of carotenes and Chl a in photosynthesizing leaves and indicate distinct acclimatory responses of their turnover to changing irradiance. In addition, the results are discussed in the context of photosystem II repair cycle and D1 protein turnover.
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