ArticleThe Plant cell2010
The {gamma}-tubulin complex protein GCP4 is required for organizing functional microtubule arrays in Arabidopsis thaliana.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 83 citations in OpenAlex.
- Cellular dynamics and molecular signaling networks of plant cytokinesis.Molecules and cells · 2026Review
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- The microtubule-nucleating factor MACERATOR tethers AUGMIN7 to microtubules and governs phragmoplast architecture.The Plant cell · 2024Article
- Cortical microtubules contribute to division plane positioning during telophase in maize.The Plant cell · 2023Article
- Brassinosteroid signals cooperate with katanin-mediated microtubule severing to control stamen filament elongation.The EMBO journal · 2023Article
- How Microtubules Build the Spindle Branch by Branch.Annual review of cell and developmental biology · 2022Review
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis.Frontiers in plant science · 2022Article
- Comparative transcriptomics analysis of developing peanut (Frontiers in plant science · 2022Article
- Two Kinesin-14A Motors Oligomerize to Drive Poleward Microtubule Convergence for Acentrosomal Spindle Morphogenesis inFrontiers in cell and developmental biology · 2022Article
- Micron-scale geometrical features of microtubules as regulators of microtubule organization.eLife · 2021Review
- Article
- γ-Tubulin Complexes and Fibrillar Arrays: Two Conserved High Molecular Forms with Many Cellular Functions.Cells · 2021Review
- Choreography of the centrosome.Heliyon · 2020Review
- OsMTOPVIB is required for meiotic bipolar spindle assembly.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Phragmoplast microtubule dynamics - a game of zones.Journal of cell science · 2018Review
- Article
- Update: Plant Cortical Microtubule Arrays.Plant physiology · 2018Review
- Comparative transcriptomic analysis of the evolution and development of flower size in Saltugilia (Polemoniaceae).BMC genomics · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule (MT) nucleation and organization depend on the evolutionarily conserved protein gamma -tubulin, which forms a complex with GCP2-GCP6 (GCP for gamma -Tubulin Complex Protein). To date, it is still unclear how GCP4-GCP6 (the non-core GCPs) may be involved in acentrosomal MT nucleation in plant cells. We found that GCP4 was associated with gamma -tubulin in vivo in Arabidopsis thaliana. When GCP4 expression was repressed by an artificial microRNA, transgenic plants exhibited phenotypes of dwarfism and reduced organ size. In mitotic cells, it was observed that the gamma -tubulin signal associated with the mitotic spindle, and the phragmoplast was depleted when GCP4 was downregulated. Consequently, MTs failed to converge at unified spindle poles, and the bipolar phragmoplast MT array frequently had discrete bundles with extended minus ends, resulting in failed cytokinesis as reflected by cell wall stubs in leaf epidermal cells. In addition, cortical MTs in swollen guard cells and pavement cells of the leaf epidermis became hyperparallel and bundled, which was likely caused by frequent MT nucleation with shallow angles on the wall of extant MTs. Therefore, our results support the notion that GCP4 is an indispensable component for the function of gamma -tubulin in MT nucleation and organization in plant cells.
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