ReviewMethods (San Diego, Calif.)2010
Live-cell analysis of kinetochore-microtubule interaction in budding yeast.
Review in Methods (San Diego, Calif.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Role of Nuclear SUMO-Targeted Ubiquitin Ligase Uls2 (Slx5-Slx8) in Quality Control of Cytosolic Proteins.Biomolecules · 2026Article
- Nuclear division phenotypes in Sporidiobolales and Trichosporonales.Microbiology spectrum · 2025Article
- Swap and stop - Kinetochores play error correction with microtubules: Mechanisms of kinetochore-microtubule error correction: Mechanisms of kinetochore-microtubule error correction.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Potassium starvation induces autophagy in yeast.The Journal of biological chemistry · 2020Article
- Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.The Journal of biological chemistry · 2019Article
- Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast.Current biology : CB · 2019Article
- Article
- Unattached kinetochores drive their own capturing by sequestering a CLASP.Nature communications · 2018Article
- Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase.Molecular cell · 2017Article
- Mechanisms mitigating problems associated with multiple kinetochores on one microtubule in early mitosis.Journal of cell science · 2017Article
- Molecular mechanisms facilitating the initial kinetochore encounter with spindle microtubules.The Journal of cell biology · 2017Article
- Dual mechanisms regulate the recruitment of spindle assembly checkpoint proteins to the budding yeast kinetochore.Molecular biology of the cell · 2016Article
- Oriented cell division: new roles in guiding skin wound repair and regeneration.Bioscience reports · 2015Review
- A comprehensive model to predict mitotic division in budding yeasts.Molecular biology of the cell · 2015Article
- Kinetochore-microtubule error correction is driven by differentially regulated interaction modes.Nature cell biology · 2015Article
- An array of nuclear microtubules reorganizes the budding yeast nucleus during quiescence.The Journal of cell biology · 2013Article
- Ordered kinetochore assembly in the human-pathogenic basidiomycetous yeast Cryptococcus neoformans.mBio · 2013Article
- Stochastic association of neighboring replicons creates replication factories in budding yeast.The Journal of cell biology · 2013Article
- Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion.Eukaryotic cell · 2012Article
- A coordinated interdependent protein circuitry stabilizes the kinetochore ensemble to protect CENP-A in the human pathogenic yeast Candida albicans.PLoS genetics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Kinetochore capture and transport by spindle microtubules plays a crucial role in high-fidelity chromosome segregation, although its detailed mechanism has remained elusive. It has been difficult to observe individual kinetochore-microtubule interactions because multiple kinetochores are captured by microtubules during a short period within a small space. We have developed a method to visualize individual kinetochore-microtubule interactions in Saccharomyces cerevisiae, by isolating one of the kinetochores from others through regulation of the activity of a centromere. We detail this technique, which we call 'centromere reactivation system', for dissection of the process of kinetochore capture and transport on mitotic spindle. Kinetochores are initially captured by the side of microtubules extending from a spindle pole, and subsequently transported poleward along them, which is an evolutionarily conserved process from yeast to vertebrate cells. Our system, in combination with amenable yeast genetics, has proved useful to elucidate the molecular mechanisms of kinetochore-microtubule interactions. We discuss practical considerations for applying our system to live cell imaging using fluorescence microscopy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.