ArticleBMC microbiology2010
Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 78 citations in OpenAlex.
- Glutamine from plasma-like medium fuels nitrogen metabolism and influences proliferation in Leptospira interrogans.PLoS pathogens · 2026Article
- TheInfection and immunity · 2026Article
- DMEM and EMEM as alternate growth media for pathogenic Leptospira.PLoS neglected tropical diseases · 2026Article
- Cultivation of Leptospira interrogans Within Rat Peritoneal Dialysis Membrane Chambers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Divergent Fates of Hardjo Leptospires: Early Transcriptomic Response ofTransboundary and emerging diseases · 2026Article
- The Single-Step Method of RNA Purification Applied to Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Alternative Sigma Factors of RNA Polymerase as Master Regulators in the Pathogenic SpirochaetePathogens (Basel, Switzerland) · 2025Review
- Evidence for a Putative Regulatory System Consisting of an ECF σInternational journal of molecular sciences · 2025Article
- DMEM and EMEM are suitable surrogate media to mimic host environment and expand leptospiral pathogenesis studies usingbioRxiv : the preprint server for biology · 2025Article
- Evolutionary insights into the emergence of virulent Leptospira spirochetes.PLoS pathogens · 2024Article
- Evolutionary insights into the emergence of virulentbioRxiv : the preprint server for biology · 2024Article
- Immunoreactivity of a Putative ECF σ Factor, LIC_10559, fromPathogens (Basel, Switzerland) · 2023Article
- Identifying Leptospira interrogans putative virulence factors with a yeast protein expression screen.Applied microbiology and biotechnology · 2022Article
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- AAA+ Molecular Chaperone ClpB inInternational journal of molecular sciences · 2020Review
- Gene expression is associated with virulence in murine macrophages infected with Leptospira spp.PloS one · 2019Article
- Article
- The sigma factor σMolecular microbiology · 2018Article
- The EbpA-RpoN Regulatory Pathway of the Pathogen Leptospira interrogans Is Essential for Survival in the Environment.Applied and environmental microbiology · 2017Article
- Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates.International journal of molecular sciences · 2017Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospirosis is a zoonosis of worldwide distribution caused by infection with pathogenic serovars of Leptospira spp. The most common species, L. interrogans, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. Transmission of pathogenic Leptospira to humans mostly occurs through abraded skin or mucosal surfaces after direct or indirect contact with infected animals or contaminated soil or water. The spirochete then spreads hematogenously, resulting in multi-organ failure and death in severe cases. Previous DNA microarray studies have identified differentially expressed genes required for adaptation to temperature and osmolarity conditions inside the host compared to those of the environment.
resultsIn order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of L. interrogans serovar Copenhageni upon exposure to serum in comparison with EMJH medium. One hundred and sixty-eight genes were found to be differentially expressed, of which 55 were up-regulated and 113 were down-regulated. Genes of known or predicted function accounted for 54.5 and 45.1% of up- and down-regulated genes, respectively. Most of the differentially expressed genes were predicted to be involved in transcriptional regulation, translational process, two-component signal transduction systems, cell or membrane biogenesis, and metabolic pathways.
conclusionsOur study showed global transcriptional changes of pathogenic Leptospira upon exposure to serum, representing a specific host environmental cue present in the bloodstream. The presence of serum led to a distinct pattern of gene expression in comparison to those of previous single-stimulus microarray studies on the effect of temperature and osmolarity upshift. The results provide insights into the pathogenesis of leptospirosis during the early bacteremic phase of infection.
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