ArticlePloS one2010
H-independent glutamine transport in plant root tips.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Calcium and Nitrogen Availability Controls Root Exudation in Hydroponically Cultured Barley.Plant, cell & environment · 2026Article
- Cationic amino acid transporters (CAT) enhance accumulation and susceptibility to the systemic herbicide L-phosphinothricin.Nature communications · 2025Article
- Root Exudates in Soilless Culture Conditions.Plants (Basel, Switzerland) · 2025Review
- A single amino acid transporter controls the uptake of priming-inducing beta-amino acids and the associated tradeoff between induced resistance and plant growth.The Plant cell · 2022Article
- Review
- Comprehensive genomic characterization of cotton cationic amino acid transporter genes reveals that GhCAT10D regulates salt tolerance.BMC plant biology · 2022Article
- Physiological synaptic activity and recognition memory require astroglial glutamine.Nature communications · 2022Article
- Promoter profiling of Arabidopsis amino acid transporters: clues for improving crops.Plant molecular biology · 2021Review
- Tonoplast-Localized Theanine Transporter CsCAT2 May Mediate Theanine Storage in the Root of Tea Plants (Frontiers in plant science · 2021Article
- Live Imaging of Phosphate Levels in Arabidopsis Root Cells Expressing a FRET-Based Phosphate Sensor.Plants (Basel, Switzerland) · 2020Article
- Overexpression of GmAAP6a enhances tolerance to low nitrogen and improves seed nitrogen status by optimizing amino acid partitioning in soybean.Plant biotechnology journal · 2020Article
- Amino Acid Transporters in Plants: Identification and Function.Plants (Basel, Switzerland) · 2020Review
- Root Exudation of Primary Metabolites: Mechanisms and Their Roles in Plant Responses to Environmental Stimuli.Frontiers in plant science · 2019Review
- Effects of Different Chemical Forms of Nitrogen on the Quick and Reversible Inhibition of Soybean Nodule Growth and Nitrogen Fixation Activity.Frontiers in plant science · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology.eLife · 2017Article
- In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox state.Nature protocols · 2016Article
- Identification of a plastidial phenylalanine exporter that influences flux distribution through the phenylalanine biosynthetic network.Nature communications · 2015Article
- A genetically encoded Förster resonance energy transfer sensor for monitoring in vivo trehalose-6-phosphate dynamics.Analytical biochemistry · 2015Article
- A tonoplast Glu/Asp/GABA exchanger that affects tomato fruit amino acid composition.The Plant journal : for cell and molecular biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlutamine is one of the primary amino acids in nitrogen assimilation and often the most abundant amino acid in plant roots. To monitor this important metabolite, a novel genetically encoded fluorescent FRET-reporter was constructed and expressed in Arabidopsis thaliana. As a candidate for the glutamine fluxes, the root tip localized, putative amino acid transporter CAT8 was analyzed and heterologously expressed in yeast and oocytes. PRINCIPAL
findingsRapid and reversible in vivo fluorescence changes were observed in reporter-expressing root tips upon exposure and removal of glutamine. FRET changes were detected at acid and neutral pH and in the presence of a protonophore, suggesting that part of the glutamine fluxes were independent of the pH. The putative amino acid transporter CAT8 transported glutamine, had a half maximal activity at approximately 100 microM and the transport was independent of external pH. CAT8 localized not only to the plasma membrane, but additionally to the tonoplast, when tagged with GFP. Ultrastructural analysis confirmed this dual localization and additionally identified CAT8 in membranes of autophagosomes. Loss-of function of CAT8 did not affect growth in various conditions, but over-expressor plants had increased sensitivity to a structural substrate analog, the glutamine synthetase inhibitor L-methionine sulfoximine.
conclusionsThe combined data suggest that proton-independent glutamine facilitators exist in root tips.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.