ArticlePloS one2010
Characterization of Eag1 channel lateral mobility in rat hippocampal cultures by single-particle-tracking with quantum dots.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Intellectual Disability and Potassium Channelopathies: A Systematic Review.Frontiers in genetics · 2020Pooled it
- The molecular basis of KCNH1-related epileptic encephalopathy and the challenge of developing targeted therapeutics.Brain : a journal of neurology · 2026Review
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Potassium Channel KCNH1 Activating Variants Cause Altered Functional and Morphological Ciliogenesis.Molecular neurobiology · 2022Article
- Article
- Cullin 7 mediates proteasomal and lysosomal degradations of rat Eag1 potassium channels.Scientific reports · 2017Article
- Calmodulin Regulates Human Ether à Go-Go 1 (hEAG1) Potassium Channels through Interactions of the Eag Domain with the Cyclic Nucleotide Binding Homology Domain.The Journal of biological chemistry · 2016Article
- Surface dynamics of voltage-gated ion channels.Channels (Austin, Tex.) · 2016Review
- Cyclic expression of the voltage-gated potassium channel KV10.1 promotes disassembly of the primary cilium.EMBO reports · 2016Article
- De novo KCNH1 mutations in four patients with syndromic developmental delay, hypotonia and seizures.Journal of human genetics · 2016Review
- Kv10.1 K(+) channel: from physiology to cancer.Pflugers Archiv : European journal of physiology · 2016Review
- KV 10.1 opposes activity-dependent increase in Ca²⁺ influx into the presynaptic terminal of the parallel fibre-Purkinje cell synapse.The Journal of physiology · 2015Article
- Positive correlation between the expression of hEag1 and HIF-1α in breast cancers: an observational study.BMJ open · 2014Observational
- The punctate localization of rat Eag1 K+ channels is conferred by the proximal post-CNBHD region.BMC neuroscience · 2014Article
- Intracellular calcium stores drive slow non-ribbon vesicle release from rod photoreceptors.Frontiers in cellular neuroscience · 2014Article
- Behavioural and functional characterization of Kv10.1 (Eag1) knockout mice.Human molecular genetics · 2013Article
- Human glioma-initiating cells show a distinct immature phenotype resembling but not identical to NG2 glia.Journal of neuropathology and experimental neurology · 2013Article
- Flavonoid regulation of EAG1 channels.The Journal of general physiology · 2013Article
- Tracking quantum dot-tagged calcium channels at vertebrate photoreceptor synapses: retinal slices and dissociated cells.Current protocols in neuroscience · 2013Article
- Lateral mobility of L-type calcium channels in synaptic terminals of retinal bipolar cells.Molecular vision · 2013Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Voltage-gated ion channels are main players involved in fast synaptic events. However, only slow intracellular mechanisms have so far been described for controlling their localization as real-time visualization of endogenous voltage-gated channels at high temporal and spatial resolution has not been achieved yet. Using a specific extracellular antibody and quantum dots we reveal and characterize lateral mobility as a faster mechanism to dynamically control the number of endogenous ether-a-go-go (Eag)1 ion channels inside synapses. We visualize Eag1 entering and leaving synapses by lateral diffusion in the plasma membrane of rat hippocampal neurons. Mathematical analysis of their trajectories revealed how the motion of Eag1 gets restricted when the channels diffuse into the synapse, suggesting molecular interactions between Eag1 and synaptic components. In contrast, Eag1 channels switch to Brownian movement when they exit synapses and diffuse into extrasynaptic membranes. Furthermore, we demonstrate that the mobility of Eag1 channels is specifically regulated inside synapses by actin filaments, microtubules and electrical activity. In summary, using single-particle-tracking techniques with quantum dots nanocrystals, our study shows for the first time the lateral diffusion of an endogenous voltage-gated ion channel in neurons. The location-dependent constraints imposed by cytoskeletal elements together with the regulatory role of electrical activity strongly suggest a pivotal role for the mobility of voltage-gated ion channels in synaptic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.