ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2010
Stress granule formation mediates the inhibition of colonic Hsp70 translation by interferon-gamma and tumor necrosis factor-alpha.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 38 citations in OpenAlex.
- Biogenesis of TNF-α-insights into proteostasis and inflammation.The FEBS journal · 2026Review
- Stress granule dynamics orchestrate colitis-to-cancer transition via TOM1-mediated disassembly and driven oncogenesis in response to inflammatory oxidative stress.Redox biology · 2026Article
- Unveiling novel insights into the role and characteristics of sperm heat shock protein 70 in indigenous Donggala bulls: implications for molecular-based motility markers.Archives animal breeding · 2026Article
- Design of CellProfiler-Based Pipelines Enabling the Attribution of Molecular Stress Markers to Specific Tissue and Subcellular Compartments of the Colonic Mucosa.Cellular and molecular gastroenterology and hepatology · 2026Article
- Organization of the Proteostasis Network of Membraneless Organelles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Connecting the Dots: Stress Granule and Cardiovascular Diseases.Journal of cardiovascular translational research · 2025Review
- Neuroimmune signaling mediates astrocytic nucleocytoplasmic disruptions and stress granule formation associated with TDP-43 pathology.Neurobiology of disease · 2025Article
- Aggregation of Stress Granules Induced by Hypoxia and Lipopolysaccharide via PKR-p-eIF2α Pathway and 4EBP1 Pathway Inhibits the Inflammatory Response in Peri-Implantitis.Journal of inflammation research · 2025Article
- Long way up: rethink diseases in light of phase separation and phase transition.Protein & cell · 2024Review
- FAM69C functions as a kinase for eIF2α and promotes stress granule assembly.EMBO reports · 2023Article
- Stress granules: potential therapeutic targets for infectious and inflammatory diseases.Frontiers in immunology · 2023Review
- Cromolyn platform suppresses fibrosis and inflammation, promotes microglial phagocytosis and neurite outgrowth.Scientific reports · 2021Article
- Article
- Assembly of Cytoplasmic Stress Granules in Placentas in Women with Preeclampsia.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Pro-Inflammatory Cytokines and Antibodies Induce hnRNP A1 Dysfunction in Mouse Primary Cortical Neurons.Brain sciences · 2021Article
- Response to stress in biological disorders: Implications of stress granule assembly and function.Cell proliferation · 2021Review
- Heat shock proteins-driven stress granule dynamics: yet another avenue for cell survival.Apoptosis : an international journal on programmed cell death · 2021Review
- Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.Antiviral research · 2021Article
- Polyhexamethylene guanidine phosphate, chloromethylisothiazolinone, and particulate matter are dispensable for stress granule formation in human airway epithelial cells.Animal cells and systems · 2021Article
- Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mucosal inflammation, through cytokines such as interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha), has many effects on the intestinal epithelium, including selective translational inhibition of the cytoprotective protein heat shock protein 70 (Hsp70). To further elucidate the mechanisms underlying this effect, we examined the role of stress granules in mediating the actions of these proinflammatory cytokines. Using conditionally immortalized young adult mouse colonic epithelial cells, we demonstrate that IFN-gamma and TNF-alpha, which upregulate eukaryotic initiation factor-alpha (eIF-2alpha) phosphorylation and reduce Hsp70 translation, significantly enhance stress granule formation in heat-shocked intestinal epithelial cells. The IFN-gamma and TNF-alpha effects in upregulation of stress granule formation and downregulation of Hsp70 were eIF-2alpha dependent, and the effect could be negated by blocking eIF-2alpha phosphorylation with use of an RNA-dependent protein kinase inhibitor. Correspondingly, IFN-gamma and TNF-alpha increased binding of cytoplasmic proteins to the 3'-untranslated region of Hsp70 mRNA, suggesting specific recruitment of Hsp70 to stress granules as the mechanism of IFN-gamma and TNF-alpha inhibition of Hsp70 translation. We thus report a novel linkage between inflammatory cytokine production, stress granule formation, and Hsp70 translation inhibition, providing additional insights into the response of intestinal epithelial cells to inflammatory stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.