ArticleThe Journal of molecular diagnostics : JMD2010
Development and evaluation of a multiplex real-time polymerase chain reaction procedure to clinically type prevalent Salmonella enterica serovars.
Article in The Journal of molecular diagnostics : JMD, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Prevalence, antimicrobial resistance and detection of resistance genes of Escherichia coli and Salmonella spp. isolated from captive European wild boars reared in Brazil.Archives of microbiology · 2026Article
- Establishment of a Simple, Sensitive, and SpecificFoods (Basel, Switzerland) · 2024Article
- A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management.Veterinary medicine (Auckland, N.Z.) · 2024Review
- An outbreak of Salmonella enterica subsp. enterica serovar Give associated with foodborne illness in the department of Vichada, Colombia, 2015Biomedica : revista del Instituto Nacional de Salud · 2021Article
- Rapid Detection and Differentiating of the PredominantFrontiers in cellular and infection microbiology · 2021Article
- Systematic Evaluation of Whole Genome Sequence-Based Predictions ofFrontiers in microbiology · 2020Article
- Multiplex ligation reaction based on probe melting curve analysis: a pragmatic approach for the identification of 30 common Salmonella serovars.Annals of clinical microbiology and antimicrobials · 2019Article
- Simultaneous Identification of Clinically CommonFrontiers in cellular and infection microbiology · 2019Article
- Salmonella in Dairy Cattle.The Veterinary clinics of North America. Food animal practice · 2018Review
- Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples.Applied and environmental microbiology · 2017Article
- Article
- The use of factorial design, image analysis, and an efficiency calculation for multiplex PCR optimization.Journal of clinical laboratory analysis · 2013Article
- One-step triplex high-resolution melting analysis for rapid identification and simultaneous subtyping of frequently isolated Salmonella serovars.Applied and environmental microbiology · 2012Article
- Article
- Rapid genoserotyping tool for classification of Salmonella serovars.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A multiplex real-time polymerase chain reaction procedure was developed to identify the most prevalent clinical isolates of Salmonella enterica subsp. enterica. Genes from the rfb, fliC, fljB, and viaB groups that encode the O, H, and Vi antigens were used to design 15 primer pairs and TaqMan probes specific for the genes rfbJ, wzx, fliC, fljB, wcdB, the sdf-l sequence, and invA, which was used as an internal amplification control. The primers and probes were variously combined into six sets. The first round of reactions used two of these sets to detect Salmonella O:4, O:9, O:7, O:8, and O:3,10 serogroups. Once the serogroups were identified, the results of a second round of reactions that used primers and probes for the flagellar antigen l genes, 1,2; e,h; g,m; d; e,n,x; and z(10), and the Vi gene were used to identify individual serovars. The procedure was standardized using 18 Salmonella reference strains and other enterobacteria. The procedure's reliability and sensitivity was evaluated using 267 randomly chosen serotyped Salmonella clinical isolates. The procedure had a sensitivity of 95.5% and was 100% specific. Thus, our technique is a quick, sensitive, reliable, and specific means of identifying S. enterica serovars and can be used in conjunction with traditional serotyping. Other primer and probe combinations could be used to increase the number of identifiable serovars.
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