ArticleDevelopment (Cambridge, England)2010
Repression of zygotic gene expression in the Xenopus germline.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- A new atlas to study embryonic cell types in Xenopus.Developmental biology · 2024Article
- Functional annotation of a hugely expanded nanos repertoire in Lytechinus variegatus, the green sea urchin.Molecular reproduction and development · 2023Article
- RNA localization during early development of the axolotl.Frontiers in cell and developmental biology · 2023Article
- Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.Nature communications · 2022Article
- Review
- The functional diversity of the POUV-class proteins across vertebrates.Open biology · 2022Review
- Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?Biology open · 2021Article
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- The POU Transcription Factor POU-M2 RegulatesGenes · 2020Article
- Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline.PLoS genetics · 2019Article
- The Effect of an Environmentally Relevant Phthalate Mixture on Primordial Germ Cells ofmicroPublication biology · 2018Article
- A novel role forDevelopment (Cambridge, England) · 2018Article
- Article
- Role of maternal Xenopus syntabulin in germ plasm aggregation and primordial germ cell specification.Developmental biology · 2017Article
- Maternal Dead-end 1 promotes translation ofDevelopment (Cambridge, England) · 2017Article
- High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration.Development (Cambridge, England) · 2017Article
- Transcription factors Mix1 and VegT, relocalization of vegt mRNA, and conserved endoderm and dorsal specification in frogs.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Primordial germ cells: the first cell lineage or the last cells standing?Development (Cambridge, England) · 2015Review
- Dynamic intracellular localization of Dazl protein during Xenopus germline development.Histochemistry and cell biology · 2015Article
- The Xenopus Maternal-to-Zygotic Transition from the Perspective of the Germline.Current topics in developmental biology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Primordial germ cells (PGCs) in Xenopus are specified through the inheritance of germ plasm. During gastrulation, PGCs remain totipotent while surrounding cells in the vegetal mass become committed to endoderm through the action of the vegetal localized maternal transcription factor VegT. We find that although PGCs contain maternal VegT RNA, they do not express its downstream targets at the mid-blastula transition (MBT). Transcriptional repression in PGCs correlates with the failure to phosphorylate serine 2 in the carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (RNAPII). As serine 5 is phosphorylated, these results are consistent with a block after the initiation step but before the elongation step of RNAPII-based transcription. Repression of PGC gene expression occurs despite an apparently permissive chromatin environment. Phosphorylation of CTD-serine 2 and expression of zygotic mRNAs in PGCs are first detected at neurula, some 10 hours after MBT, indicating that transcription is significantly delayed in the germ cell lineage. Significantly, Oct-91, a POU subclass V transcription factor related to mammalian Oct3/4, is among the earliest zygotic transcripts detected in PGCs and is a likely mediator of pluripotency. Our findings suggest that PGCs are unable to respond to maternally inherited endoderm determinants because RNAPII activity is transiently blocked while these determinants are present. Our results in a vertebrate system further support the concept that one strategy used repeatedly during evolution for preserving the germline is RNAPII repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.