ArticleNucleic acids research2010
NMR structure and dynamics of the Specifier Loop domain from the Bacillus subtilis tyrS T box leader RNA.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Targeting Gene Transcription Prevents Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2025Review
- Another layer of complexity in Staphylococcus aureus methionine biosynthesis control: unusual RNase III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay.Nucleic acids research · 2021Article
- An evolving tale of two interacting RNAs-themes and variations of the T-box riboswitch mechanism.IUBMB life · 2019Review
- The T-Box Riboswitch: tRNA as an Effector to Modulate Gene Regulation.Microbiology spectrum · 2018Review
- Capture and Release of tRNA by the T-Loop Receptor in the Function of the T-Box Riboswitch.Biochemistry · 2017Article
- Identification of Spermidine Binding Site in T-box Riboswitch Antiterminator RNA.Chemical biology & drug design · 2016Article
- A glyS T-box riboswitch with species-specific structural features responding to both proteinogenic and nonproteinogenic tRNAGly isoacceptors.RNA (New York, N.Y.) · 2015Article
- RNA-Puzzles Round II: assessment of RNA structure prediction programs applied to three large RNA structures.RNA (New York, N.Y.) · 2015Article
- T box riboswitches in Actinobacteria: translational regulation via novel tRNA interactions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The T box riboswitch: A novel regulatory RNA that utilizes tRNA as its ligand.Biochimica et biophysica acta · 2014Review
- Global analysis of riboswitches by small-angle X-ray scattering and calorimetry.Biochimica et biophysica acta · 2014Review
- Sequence, structure, and stacking: specifics of tRNA anchoring to the T box riboswitch.RNA biology · 2013Review
- Structural determinants for geometry and information decoding of tRNA by T box leader RNA.Structure (London, England : 1993) · 2013Article
- Solution NMR determination of hydrogen bonding and base pairing between the glyQS T box riboswitch Specifier domain and the anticodon loop of tRNA(Gly).FEBS letters · 2013Article
- Two-codon T-box riboswitch binding two tRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- T box RNA decodes both the information content and geometry of tRNA to affect gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Solution nuclear magnetic resonance analyses of the anticodon arms of proteinogenic and nonproteinogenic tRNA(Gly).Biochemistry · 2012Article
- Sequence-based identification of 3D structural modules in RNA with RMDetect.Nature methods · 2011Article
- Solution structure of the K-turn and Specifier Loop domains from the Bacillus subtilis tyrS T-box leader RNA.Journal of molecular biology · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Gram-positive bacteria utilize a tRNA-responsive transcription antitermination mechanism, designated the T box system, to regulate expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes. The RNA transcripts of genes controlled by this mechanism contain 5' untranslated regions, or leader RNAs, that specifically bind cognate tRNA molecules through pairing of nucleotides in the tRNA anticodon loop with nucleotides in the Specifier Loop domain of the leader RNA. We have determined the solution structure of the Specifier Loop domain of the tyrS leader RNA from Bacillus subtilis. Fifty percent of the nucleotides in the Specifier Loop domain adopt a loop E motif. The Specifier Sequence nucleotides, which pair with the tRNA anticodon, stack with their Watson-Crick edges rotated toward the minor groove and exhibit only modest flexibility. We also show that a Specifier Loop domain mutation that impairs the function of the B. subtilis glyQS T box RNA disrupts the tyrS loop E motif. Our results suggest a mechanism for tRNA-Specifier Loop binding in which the phosphate backbone kink created by the loop E motif causes the Specifier Sequence bases to rotate toward the minor groove, which increases accessibility for pairing with bases in the anticodon loop of tRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.