Evidence map›Paper›PMID 20109495›Full record

ArticleJournal of virological methods2010

Full length sequencing of all nine subtypes of the neuraminidase gene of influenza A viruses using subtype specific primer sets.

Yogesh Chander, Naresh Jindal, David E Stallknecht, Srinand Sreevatsan, Sagar M Goyal

Open access · greenAbstract read
In one paragraph

Article in Journal of virological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.6field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 31 citations in OpenAlex.

  1. Review
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  11. Diagnostics and surveillance for Swine influenza.Current topics in microbiology and immunology · 2013
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yogesh ChanderDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, United States.
Naresh Jindal
David E Stallknecht
Srinand Sreevatsan
Sagar M Goyal
University of Minnesota · USUniversity of Georgia · US

Funding

NIAID NIH HHS HHSN266200700007C
6 · The paper itself

Abstract

An RT-PCR based method was developed using subtype specific overlapping primers to obtain full length amplification of neuraminidase (NA) gene from all subtypes (N1-N9) of influenza A viruses. This method was validated using reference strains of avian influenza viruses (AIV) (N1-N9), human influenza viruses (N1 and N2), and swine influenza viruses (N1-N3). Amplification of the NA gene was obtained with all viruses tested. Additionally, 200 field isolates of AIV from wild birds were tested by this method and the NA gene was amplified in all isolates. The NA subtype of all 200 isolates was determined by further sequencing of the amplified NA genes and all sequences were submitted to GenBank. The method described in this paper can be used to determine subtype of influenza isolates as well as their evolution and mutations if any, in the NA gene.

Indexed as

Sequence Analysis, DNAAnimalsBirdsDNA PrimersGenotypeHumansInfluenza A virusMolecular Sequence DataNeuraminidaseReverse Transcriptase Polymerase Chain ReactionRNA, ViralSwineViral ProteinsDNA PrimersNA protein, influenza A virusNeuraminidaseRNA, ViralViral Proteins

Identifiers

PMID20109495
PMCPMC11369775
OpenAlexW2108593451

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.