ArticleJournal of immunological methods2010
Stromal cell independent B cell development in vitro: generation and recovery of autoreactive clones.
Article in Journal of immunological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed.
- The deubiquitinase activity of CYLD is required for B cell differentiation.Cell death & disease · 2026Article
- Suppression of pre-B cell colony formation by catecholamine oxidation.Journal of immunology (Baltimore, Md. : 1950) · 2026Article
- Tumor-Infiltrating B Lymphocytes: Promising Immunotherapeutic Targets for Primary Liver Cancer Treatment.Cancers · 2023Review
- Enhanced protein synthesis is a defining requirement for neonatal B cell development.Frontiers in immunology · 2023Article
- The splicing regulators TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesis.Cell reports · 2022Article
- Sin1/mTORC2 regulate B cell growth and metabolism by activating mTORC1 and Myc.Cellular & molecular immunology · 2019Article
- Identification and characterization of a murine model of BCR‑ABL1+ acute B‑lymphoblastic leukemia with central nervous system metastasis.Oncology reports · 2019Article
- SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- An essential role for the ZnNature immunology · 2019Article
- VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Activation of the MEK-ERK Pathway Is Necessary but Not Sufficient for Breaking Central B Cell Tolerance.Frontiers in immunology · 2018Article
- Enhanced antibody responses to an HIV-1 membrane-proximal external region antigen in mice reconstituted with cultured lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Article
- Generation of bone marrow and fetal liver chimeric mice.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Murine B cell development and antibody responses to model antigens are not impaired in the absence of the TNF receptor GITR.PloS one · 2012Article
- Rescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Activation-induced cytidine deaminase mediates central tolerance in B cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Role of immune mechanisms in induction of HIV-1 broadly neutralizing antibodies.Current opinion in immunology · 2011Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe and characterize a stromal cell independent culture system that efficiently supports pro-B cell to IgM+ B cell development with near normal levels of IgH and Igkappa diversity. Pro-B cells present in non-adherent bone marrow cells proliferate in the presence of IL-7 and subsequent to the removal of IL-7 and addition of BAFF, differentiate normally into IgM+ B cells. B cell development in vitro closely follows the patterns of development in vivo with culture-derived (CD) B cells demonstrating characteristic patterns of surface antigen expression and gene activation. IgM+ CD B cells respond to TLR stimulation by proliferation and differentiation into antibody-secreting cells. Self-reactive IgM+ B cell development is blocked in 3H9 IgH knockin mice; however, cultures of 3H9 IgH knockin pro-B cells yields high frequencies of "forbidden", autoreactive IgM+ B cells. Furthermore, serum IgG autoantibody exceeded that present in autoimmune, C4(-/-) animals following the reconstitution of RAG1(-/-) mice with IgM+ CD cells derived from BL/6 mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.