ArticleBMC biochemistry2010
Rapid determination of tricarboxylic acid cycle enzyme activities in biological samples.
Article in BMC biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
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- Mitochondrial CaMKII causes adverse metabolic reprogramming and dilated cardiomyopathy.Nature communications · 2020Article
- Novel ginsenoside derivative 20(S)-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism.Cell death & disease · 2020Article
- Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.Molecular genetics and metabolism · 2019Article
- Article
- Bovine and murine models highlight novel roles for SLC25A46 in mitochondrial dynamics and metabolism, with implications for human and animal health.PLoS genetics · 2017Article
- Mitochondrial Probe Methyltriphenylphosphonium (TPMP) Inhibits the Krebs Cycle Enzyme 2-Oxoglutarate Dehydrogenase.PloS one · 2016Article
- The effect of Walterinnesia aegyptia venom proteins on TCA cycle activity and mitochondrial NAD(+)-redox state in cultured human fibroblasts.BioMed research international · 2015Article
- S-glutathionylation reactions in mitochondrial function and disease.Frontiers in cell and developmental biology · 2014Review
- Resveratrol induces a mitochondrial complex I-dependent increase in NADH oxidation responsible for sirtuin activation in liver cells.The Journal of biological chemistry · 2013Article
- Murine models and cell lines for the investigation of pheochromocytoma: applications for future therapies?Endocrine pathology · 2012Review
- Prediction of gastric cancer metastasis through urinary metabolomic investigation using GC/MS.World journal of gastroenterology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the last ten years, deficiencies in tricarboxylic acid cycle (TCAC) enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological and cardiac diseases. A prerequisite to the identification of disease-causing TCAC enzyme deficiencies is the availability of effective enzyme assays.
resultsWe developed three assays that measure the full set of TCAC enzymes. One assay relies on the sequential addition of reagents to measure succinyl-CoA ligase activity, followed by succinate dehydrogenase, fumarase and, finally, malate dehydrogenase. Another assay measures the activity of alpha-ketoglutarate dehydrogenase followed by aconitase and isocitrate dehydrogenase. The remaining assay measures citrate synthase activity using a standard procedure. We used these assays successfully on extracts of small numbers of human cells displaying various severe or partial TCAC deficiencies and on frozen heart homogenates from heterozygous mice harboring an SDHB gene deletion.
conclusionThis set of assays is rapid and simple to use and can immediately detect even partial defects, as the activity of each enzyme can be readily compared with one or more other activities measured in the same sample.
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