ArticlePLoS genetics2010
Nonsense-mediated decay enables intron gain in Drosophila.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Quantitative RNA-seq meta-analysis of alternative exon usage inGenome research · 2017Pooled it
- De Novo Creation of Two Novel Spliceosomal Introns of RECG1 by Intronization of Formerly Exonic Sequences in Orchidaceae.Journal of molecular evolution · 2025Article
- Intronization Signatures in Coding Exons Reveal the Evolutionary Fluidity of Eukaryotic Gene Architecture.Microorganisms · 2022Article
- Intron losses and gains in the nematodes.Biology direct · 2022Article
- Gene expression and alternative splicing dynamics are perturbed in female head transcriptomes following heterospecific copulation.BMC genomics · 2021Article
- Rapidly evolving protointrons in Saccharomyces genomes revealed by a hungry spliceosome.PLoS genetics · 2019Article
- Exosome-mediated horizontal gene transfer occurs in double-strand break repair during genome editing.Communications biology · 2019Article
- Refining the Ambush Hypothesis: Evidence That GC- and AT-Rich Bacteria Employ Different Frameshift Defence Strategies.Genome biology and evolution · 2018Article
- The evolution and diversity of the nonsense-mediated mRNA decay pathway.F1000Research · 2018Review
- Functional Characterization of SMG7 Paralogs inFrontiers in plant science · 2018Article
- Adenine Enrichment at the Fourth CDS Residue in Bacterial Genes Is Consistent with Error Proofing for +1 Frameshifts.Molecular biology and evolution · 2017Article
- Nonsense-mediated mRNA decay in Tetrahymena is EJC independent and requires a protozoa-specific nuclease.Nucleic acids research · 2017Article
- Splice Sites Seldom Slide: Intron Evolution in Oomycetes.Genome biology and evolution · 2016Article
- Current Bacterial Gene Encoding Capsule Biosynthesis Protein CapI Contains Nucleotides Derived from Exonization.Evolutionary bioinformatics online · 2016Article
- Imprecise intron losses are less frequent than precise intron losses but are not rare in plants.Biology direct · 2015Article
- Intron evolution in Neurospora: the role of mutational bias and selection.Genome research · 2015Article
- Recurrent loss of specific introns during angiosperm evolution.PLoS genetics · 2014Article
- Characterization of newly gained introns in Daphnia populations.Genome biology and evolution · 2014Article
- Article
- Frequency of intron loss correlates with processed pseudogene abundance: a novel strategy to test the reverse transcriptase model of intron loss.BMC biology · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Intron number varies considerably among genomes, but despite their fundamental importance, the mutational mechanisms and evolutionary processes underlying the expansion of intron number remain unknown. Here we show that Drosophila, in contrast to most eukaryotic lineages, is still undergoing a dramatic rate of intron gain. These novel introns carry significantly weaker splice sites that may impede their identification by the spliceosome. Novel introns are more likely to encode a premature termination codon (PTC), indicating that nonsense-mediated decay (NMD) functions as a backup for weak splicing of new introns. Our data suggest that new introns originate when genomic insertions with weak splice sites are hidden from selection by NMD. This mechanism reduces the sequence requirement imposed on novel introns and implies that the capacity of the spliceosome to recognize weak splice sites was a prerequisite for intron gain during eukaryotic evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.