ArticlePloS one2010
Temporal and spatial requirements of unplugged/MuSK function during zebrafish neuromuscular development.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Dynamic expression of the zebrafish (Danio rerio) proteome across early larval development.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2025Article
- Quantification of Neuromuscular Junctions in Zebrafish Cranial Muscles.Bio-protocol · 2025Article
- NMJ Analyser: a novel method to quantify neuromuscular junction morphology in zebrafish.Bioinformatics (Oxford, England) · 2023Article
- Embryonic ethanol exposure disrupts craniofacial neuromuscular integration in zebrafish larvae.Frontiers in physiology · 2023Article
- Development of a high-throughput tailored imaging method in zebrafish to understand and treat neuromuscular diseases.Frontiers in molecular neuroscience · 2022Article
- Agrin/Lrp4 signal constrains MuSK-dependent neuromuscular synapse development in appendicular muscle.Development (Cambridge, England) · 2021Article
- The beta-adrenergic agonist salbutamol modulates neuromuscular junction formation in zebrafish models of human myasthenic syndromes.Human molecular genetics · 2018Article
- Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori.International journal of molecular sciences · 2018Article
- Article
- HnRNP C, YB-1 and hnRNP L coordinately enhance skipping of human MUSK exon 10 to generate a Wnt-insensitive MuSK isoform.Scientific reports · 2014Article
- LRP4 is critical for neuromuscular junction maintenance.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Development of oculomotor circuitry independent of hox3 genes.Nature communications · 2014Article
- Conservation and early expression of zebrafish tyrosine kinases support the utility of zebrafish as a model for tyrosine kinase biology.Zebrafish · 2013Article
- β-Catenin stabilization in skeletal muscles, but not in motor neurons, leads to aberrant motor innervation of the muscle during neuromuscular development in mice.Developmental biology · 2012Article
- Initiation of synapse formation by Wnt-induced MuSK endocytosis.Development (Cambridge, England) · 2012Article
- Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.American journal of human genetics · 2011Article
- Novel mouse model reveals distinct activity-dependent and -independent contributions to synapse development.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
One of the earliest events in neuromuscular junction (NMJ) development is the accumulation of acetylcholine receptor (AChR) at the center of muscle cells. The unplugged/MuSK (muscle specific tyrosine kinase) gene is essential to initiate AChR clustering but also to restrict approaching growth cones to the muscle center, thereby coordinating pre- and postsynaptic development. To determine how unplugged/MuSK signaling coordinates these two processes, we examined the temporal and spatial requirements of unplugged/MuSK in zebrafish embryos using heat-shock inducible transgenes. Here, we show that despite its expression in muscle cells from the time they differentiate, unplugged/MuSK activity is first required just prior to the appearance of AChR clusters to simultaneously induce AChR accumulation and to guide motor axons. Furthermore, we demonstrate that ectopic expression of unplugged/MuSK throughout the muscle membrane results in wildtype-like AChR prepattern and neuromuscular synapses in the central region of muscle cells. We propose that AChR prepatterning and axonal guidance are spatio-temporally coordinated through common unplugged/MuSK signals, and that additional factor(s) restrict unplugged/MuSK signaling to a central muscle zone critical for establishing mid-muscle synaptogenesis.
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