ArticleThe Journal of investigative dermatology2010
Dynamic relationship of focal contacts and hemidesmosome protein complexes in live cells.
Article in The Journal of investigative dermatology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Intracellular TAS2Rs act as a gatekeeper for the excretion of harmful substances via ABCB1 in keratinocytes.FASEB bioAdvances · 2024Article
- Regulation of Kinase Signaling Pathways by α6β4-Integrins and Plectin in Prostate Cancer.Cancers · 2022Review
- Loss of α6β4 Integrin-Mediated Hemidesmosomes Promotes Prostate Epithelial Cell Migration by Stimulating Focal Adhesion Dynamics.Frontiers in cell and developmental biology · 2022Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- Hemidesmosomes modulate force generation via focal adhesions.The Journal of cell biology · 2020Article
- The spectraplakin Dystonin antagonizes YAP activity and suppresses tumourigenesis.Scientific reports · 2019Article
- Bullous Pemphigoid IgG Induces Cell Dysfunction and Enhances the Motility of Epidermal Keratinocytes via Rac1/Proteasome Activation.Frontiers in immunology · 2019Article
- Mouse Keratinocytes Without Keratin Intermediate Filaments Demonstrate Substrate Stiffness Dependent Behaviors.Cellular and molecular bioengineering · 2018Article
- Loss of MMP-8 in ductal carcinoma in situ (DCIS)-associated myoepithelial cells contributes to tumour promotion through altered adhesive and proteolytic function.Breast cancer research : BCR · 2017Article
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Molecular architecture and function of the hemidesmosome.Cell and tissue research · 2015Review
- Alpha actinin-1 regulates cell-matrix adhesion organization in keratinocytes: consequences for skin cell motility.The Journal of investigative dermatology · 2015Article
- The role of hemidesmosomes and focal contacts in the skin visualized by dual-color live cell imaging.Medical molecular morphology · 2014Review
- Focal Contact and Hemidesmosomal Proteins in Keratinocyte Migration and Wound Repair.Advances in wound care · 2014Review
- Plectin-containing, centrally localized focal adhesions exert traction forces in primary lung epithelial cells.Journal of cell science · 2013Article
- Plectin-intermediate filament partnership in skin, skeletal muscle, and peripheral nerve.Histochemistry and cell biology · 2013Review
- Bullous pemphigoid IgG induces BP180 internalization via a macropinocytic pathway.The American journal of pathology · 2013Article
- Actinin-4 in keratinocytes regulates motility via an effect on lamellipodia stability and matrix adhesions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Keratins mediate localization of hemidesmosomes and repress cell motility.The Journal of investigative dermatology · 2013Article
- Skin basement membrane: the foundation of epidermal integrity--BM functions and diverse roles of bridging molecules nidogen and perlecan.BioMed research international · 2013Review
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Epidermal cells adhere to the basement membrane zone through cell-matrix junctions termed hemidesmosomes. During wound healing, hemidesmosomes are disassembled to allow keratinocytes to move over wound sites. Such movement is mediated by both hemidesmosome protein complexes (HPCs) and focal contacts (FCs). In this study, we analyzed the interaction between HPCs and FCs in live HaCat cells expressing yellow fluorescent protein (YFP)-tagged beta4 integrin and cyan fluorescent protein (CFP)-tagged alpha-actinin as markers of HPCs and FCs, respectively. In HaCat cells migrating to repopulate wounds, FC proteins cluster rapidly in the direction of the wound. HPC assembly then follows and the newly formed HPCs occupy sites vacated by the disassembled FCs. HPC dynamics are dramatically reduced, and HaCat cells cease migration upon treatment with reagents that affect FC integrity/function. Upon treatment with reagents that destabilize HPCs, the dynamics of FCs in HaCat cells at the edges of wounds are enhanced, although FC assembly is irregular and the migration of the cells is aberrant. We also show that the complex interaction between hemidesmosomes and FCs in keratinocytes is myosin dependent and requires energy. In summary, we suggest that HPCs and FCs dynamics are tightly co-regulated in keratinocytes undergoing migration during wound healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.