ArticleClinical and vaccine immunology : CVI2010
Diagnosing human anisakiasis: recombinant Ani s 1 and Ani s 7 allergens versus the UniCAP 100 fluorescence enzyme immunoassay.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Anisakis sensitization in different population groups and public health impact: A systematic review.PloS one · 2018Pooled it
- Anisakiasis: A Decade of Molecular and Diagnostic Advancements (2015-2026).International journal of molecular sciences · 2026Review
- Zoonotic Marine Nematode Infection of Fish Products in Landlocked Country, Slovakia.Emerging infectious diseases · 2023Article
- Analysis of Ani s 7 and Ani s 1 allergens as biomarkers of sensitization and allergy severity in human anisakiasis.Scientific reports · 2020Article
- Urticaria and silent parasitism by Ascaridoidea: Component-resolved diagnosis reinforces the significance of this association.PLoS neglected tropical diseases · 2020Article
- Anisakis Sensitization in the Croatian fish processing workers: Behavioral instead of occupational risk factors?PLoS neglected tropical diseases · 2020Article
- Comparison of recombinant cathepsins L1, L2, and L5 as ELISA targets for serodiagnosis of bovine and ovine fascioliasis.Parasitology research · 2018Article
- Excretory/secretory products of anisakid nematodes: biological and pathological roles.Acta veterinaria Scandinavica · 2017Review
- New Perspectives on the Diagnosis of Allergy to Anisakis spp.Current allergy and asthma reports · 2017Review
- Anisakiasis Causing Acute Dysentery in Malaysia.The American journal of tropical medicine and hygiene · 2016Article
- Changes over Time in IgE Sensitization to Allergens of the Fish Parasite Anisakis spp.PLoS neglected tropical diseases · 2016Article
- A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient.BMC infectious diseases · 2016Article
- Anti-Anisakis IgE seroprevalence in the healthy Croatian coastal population and associated risk factors.PLoS neglected tropical diseases · 2014Article
- Granulocytes in helminth infection -- who is calling the shots?Current medicinal chemistry · 2012Review
- Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.PLoS neglected tropical diseases · 2011Article
- Synergism between prior Anisakis simplex infections and intake of NSAIDs, on the risk of upper digestive bleeding: a case-control study.PLoS neglected tropical diseases · 2011Article
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Authors and funding
9 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Commercially available serological methods for serodiagnosis of human anisakiasis either are poorly specific or do not include some of the most relevant Anisakis allergens. The use of selected recombinant allergens may improve serodiagnosis. To compare the diagnostic and clinical values of enzyme-linked immunosorbent assay (ELISA) methods based on Ani s 1 and Ani s 7 recombinant allergens and of the UniCAP 100 fluorescence enzyme immunoassay (CAP FEIA) system, we tested sera from 495 allergic and 25 non-food-related allergic patients. The decay in specific IgE antibodies in serum was also investigated in 15 positive patients over a period of 6 to 38 months. Considering sera that tested positive by either Ani s 1 or Ani s 7 ELISA, the CAP FEIA classified 25% of sera as falsely positive, mainly in the group of patients with the lowest levels of anti-Anisakis IgE antibodies, and 1.28% of positive sera as falsely negative. Considering allergens individually, the overall sensitivities of Ani s 7 ELISA and Ani s 1 ELISA were 94% and 61%, respectively. The results also showed that anti-Anisakis IgE antibodies can be detected in serum for longer with Ani s 1 ELISA than with Ani s 7 ELISA and CAP FEIA (P < 0.01). Our findings suggest that ELISA methods with Ani s 7 and Ani s 1 allergens as targets of IgE antibodies are currently the best option for serodiagnosis of human anisakiasis, combining specificity and sensitivity. The different persistence of anti-Ani s 1 and anti-Ani s 7 antibodies in serum may help clinicians to distinguish between recent and old Anisakis infections.
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