ArticleClinical and vaccine immunology : CVI2010
Validation of a medium-throughput method for evaluation of intracellular growth of Mycobacterium tuberculosis.
Article in Clinical and vaccine immunology : CVI, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Adaptation of the molecular bacterial load assay for real-time quantification ofMicrobiology spectrum · 2026Article
- An HLA-E-targeted TCR bispecific molecule redirects T cell immunity against Mycobacterium tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A high-throughput screening assay based on automated microscopy for monitoring antibiotic susceptibility of Mycobacterium tuberculosis phenotypes.BMC microbiology · 2021Article
- Helminth Antigen Exposure Enhances Early Immune Control of Mycobacterium tuberculosis in Monocytes and Macrophages.Journal of innate immunity · 2021Article
- Efferocytosis of Apoptotic Neutrophils Enhances Control of Mycobacterium tuberculosis in HIV-Coinfected Macrophages in a Myeloperoxidase-Dependent Manner.Journal of innate immunity · 2020Article
- Retention of EsxA in the Capsule-Like Layer ofInfection and immunity · 2019Article
- Anti-mycobacterial activity correlates with altered DNA methylation pattern in immune cells from BCG-vaccinated subjects.Scientific reports · 2017Article
- Species dependent impact of helminth-derived antigens on human macrophages infected with Mycobacterium tuberculosis: Direct effect on the innate anti-mycobacterial response.PLoS neglected tropical diseases · 2017Article
- Inhibition of Tissue Matrix Metalloproteinases Interferes withFrontiers in microbiology · 2017Article
- Article
- The Cording Phenotype ofFrontiers in cellular and infection microbiology · 2017Article
- Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages.Scientific reports · 2016Article
- OtopathogenicFrontiers in microbiology · 2016Article
- Profiling B and T cell immune responses to co-infection of Mycobacterium tuberculosis and hookworm in humans.Infectious diseases of poverty · 2015Article
- Intra- and extracellular activities of trimethoprim-sulfamethoxazole against susceptible and multidrug-resistant Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2014Article
- Therapeutic efficacy of SQ641-NE against Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2014Article
- Replication rates of Mycobacterium tuberculosis in human macrophages do not correlate with mycobacterial antibiotic susceptibility.PloS one · 2014Article
- Apoptotic neutrophils augment the inflammatory response to Mycobacterium tuberculosis infection in human macrophages.PloS one · 2014Article
- Double recombinant Mycobacterium bovis BCG strain for screening of primary and rationale-based antimycobacterial compounds.Antimicrobial agents and chemotherapy · 2014Article
- Rapid measurement of antituberculosis drug activity in vitro and in macrophages using bioluminescence.The Journal of antimicrobial chemotherapy · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intracellular pathogens such as Mycobacterium tuberculosis have adapted to a life inside host cells, in which they utilize host nutrients to replicate and spread. Ineffective methods for the evaluation of growth of intracellular pathogens in their true environment pose an obstacle for basic research and drug screening. Here we present the validation of a luminometry-based method for the analysis of intramacrophage growth of M. tuberculosis. The method, which is performed in a medium-throughput format, can easily be adapted for studies of other intracellular pathogens and cell types. The use of host cells in drug-screening assays dedicated to find antimicrobials effective against intracellular pathogens permits the discovery of not only novel antibiotics but also compounds with immunomodulatory and virulence-impairing activities, which may be future alternatives or complements to antibiotics.
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