ArticleRNA (New York, N.Y.)2010
Two forms of RNA editing are required for tRNA maturation in Physarum mitochondria.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Article
- Review
- Article
- The Extent of mRNA Editing Is Limited in Chicken Liver and Adipose, but Impacted by Tissular Context, Genotype, Age, and Feeding as Exemplified with a Conserved Edited Site in COG3.G3 (Bethesda, Md.) · 2015Article
- Parallel Evolution and Lineage-Specific Expansion of RNA Editing in Ctenophores.Integrative and comparative biology · 2015Review
- In vitro substrate specificities of 3'-5' polymerases correlate with biological outcomes of tRNA 5'-editing reactions.FEBS letters · 2015Article
- Large gene overlaps and tRNA processing in the compact mitochondrial genome of the crustacean Armadillidium vulgare.RNA biology · 2015Article
- From end to end: tRNA editing at 5'- and 3'-terminal positions.International journal of molecular sciences · 2014Review
- Mitochondrial tRNA 5'-editing in Dictyostelium discoideum and Polysphondylium pallidum.The Journal of biological chemistry · 2014Article
- Absence of a universal element for tRNAHis identity in Acanthamoeba castellanii.Nucleic acids research · 2013Article
- Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily.RNA (New York, N.Y.) · 2012Review
- Determinants of tRNA editing and modification: avoiding conundrums, affecting function.Seminars in cell & developmental biology · 2012Review
- Complete characterization of the edited transcriptome of the mitochondrion of Physarum polycephalum using deep sequencing of RNA.Nucleic acids research · 2011Article
- A role for tRNA(His) guanylyltransferase (Thg1)-like proteins from Dictyostelium discoideum in mitochondrial 5'-tRNA editing.RNA (New York, N.Y.) · 2011Article
- tRNA 5'-end repair activities of tRNAHis guanylyltransferase (Thg1)-like proteins from Bacteria and Archaea.Nucleic acids research · 2011Article
- When you can't trust the DNA: RNA editing changes transcript sequences.Cellular and molecular life sciences : CMLS · 2011Review
- Abundant 5S rRNA-like transcripts encoded by the mitochondrial genome in amoebozoa.Eukaryotic cell · 2010Article
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Authors and funding
3 authors.
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Abstract
The mitochondrial genome of Physarum polycephalum encodes five tRNAs, four of which are edited by nucleotide insertion. Two of these tRNAs, tRNA(met1) and tRNA(met2), contain predicted mismatches at the beginning (proximal end) of the acceptor stem. In addition, the putative 5' end of tRNA(met2) overlaps the 3' end of a small, abundant, noncoding RNA, which we term ppoRNA. These anomalies led us to hypothesize that these two Physarum mitochondrial tRNAs undergo additional editing events. Here, we show that tRNA(met1) and tRNA(met2) each has a nonencoded G at its 5' end. In contrast to the other nucleotides that are added to Physarum mitochondrial RNAs, these extra G residues are likely added post-transcriptionally based on (1) the absence of added G in precursor transcripts containing inserted C and AA residues, (2) the presence of potential intermediates characteristic of 5' replacement editing, and (3) preferential incorporation of GTP into tRNA molecules under conditions that do not support transcription. This is the first report of both post-transcriptional nucleotide insertions and the addition of single Gs in P. polycephalum mitochondrial transcripts. We postulate that tRNA(met1) and tRNA(met2) are acted upon by an activity similar to that present in the mitochondria of certain other amoebozoons and chytrid fungi, suggesting that enzymes that repair the 5' end of tRNAs may be widespread.
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