ArticleBMC genomics2010
Tungsten carbide cobalt nanoparticles exert hypoxia-like effects on the gene expression level in human keratinocytes.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Research recommendations for selected IARC-classified agents.Environmental health perspectives · 2010Pooled it
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- Circular RNAs in acute myeloid leukemia.Molecular cancer · 2021Review
- Exposure-based ecotoxicity assessment of CoEnvironmental science and pollution research international · 2021Article
- Manually curated transcriptomics data collection for toxicogenomic assessment of engineered nanomaterials.Scientific data · 2021Article
- Comparative Assessment of Tungsten Toxicity in the Absence or Presence of Other Metals.Toxics · 2018Review
- Role of omics techniques in the toxicity testing of nanoparticles.Journal of nanobiotechnology · 2017Review
- An update to the toxicological profile for water-soluble and sparingly soluble tungsten substances.Critical reviews in toxicology · 2015Review
- Up-regulation of Gadd45α after exposure to metal nanoparticles: the role of hypoxia inducible factor 1α.Environmental toxicology · 2015Article
- Altered gene transcription in human cells treated with Ludox® silica nanoparticles.International journal of environmental research and public health · 2014Article
- Exploring the potential role of tungsten carbide cobalt (WC-Co) nanoparticle internalization in observed toxicity toward lung epithelial cells in vitro.Toxicology and applied pharmacology · 2014Article
- Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells.Autophagy · 2014Article
- Intracellular signal modulation by nanomaterials.Advances in experimental medicine and biology · 2014Review
- PTEN-mediated ERK1/2 inhibition and paradoxical cellular proliferation following Pnck overexpression.Cell cycle (Georgetown, Tex.) · 2014Article
- NanoMiner - integrative human transcriptomics data resource for nanoparticle research.PloS one · 2013Article
- Genotoxicity and carcinogenicity of cobalt-, nickel- and copper-based nanoparticles.Experimental and therapeutic medicine · 2012Review
- Article
- Concomitant [In vivo (Athens, Greece)Article
- Nanotoxicity: emerging concerns regarding nanomaterial safety and occupational hard metal (WC-Co) nanoparticle exposure.International journal of nanomedicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTungsten carbide (WC) and tungsten carbide cobalt (WC-Co) nanoparticles are of occupational health relevance because of the increasing usage in hard metal industries. Earlier studies showed an enhanced toxic potential for WC-Co compared to WC or cobalt ions alone. Therefore, we investigated the impact of these particles, compared to cobalt ions applied as CoCl(2), on the global gene expression level in human keratinocytes (HaCaT) in vitro.
resultsWC nanoparticles exerted very little effects on the transcriptomic level after 3 hours and 3 days of exposure. In contrast, WC-Co nanoparticles caused significant transcriptional changes that were similar to those provoked by CoCl(2). However, CoCl(2) exerted even more pronounced changes in the transcription patterns. Gene set enrichment analyses revealed that the differentially expressed genes were related to hypoxia response, carbohydrate metabolism, endocrine pathways, and targets of several transcription factors. The role of the transcription factor HIF1 (hypoxia inducible factor 1) is particularly highlighted and aspects of downstream events as well as the role of other transcription factors related to cobalt toxicity are considered.
conclusionsThis study provides extensive data useful for the understanding of nanoparticle and cobalt toxicity. It shows that WC nanoparticles caused low transcriptional responses while WC-Co nanoparticles are able to exert responses similar to that of free cobalt ions, particularly the induction of hypoxia-like effects via interactions with HIF1alpha in human keratinocytes. However, the enhanced toxicity of WC-Co particles compared to CoCl(2) could not be explained by differences in gene transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.