ArticleJournal of neurovirology2009
Advanced tracing tools: functional neuronal expression of virally encoded fluorescent calcium indicator proteins.
Article in Journal of neurovirology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- AAV9-Retro mediates efficient transduction with axon terminal absorption and blood-brain barrier transportation.Molecular brain · 2020Article
- Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.Frontiers in molecular neuroscience · 2013Article
- Microdissection of neural networks by conditional reporter expression from a Brainbow herpesvirus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Lateral Connectivity in the Olfactory Bulb is Sparse and Segregated.Frontiers in neural circuits · 2011Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Pseudorabies virus (PrV) strains such as PrV-Bartha and its marker protein-expressing variants have been used in numerous studies as retrograde transneuronal tracing tools, defining the synaptic organization of mammalian neuronal circuits. However, the possibilities for functional examination of virus-infected neurons are limited to electrophysiological approaches or bulk loading strategies using calcium-sensitive dyes. Herein we report the generation and functional characterization of three PrV-Bartha-derived recombinant virus mutants that express different fluorescent calcium indicator proteins (FCIPs). All three generated virus recombinants are able to infect murine trigeminal neurons and express the corresponding FCIP (GCaMP2, camgaroo-2, or inverse pericam). Functionality of these virally expressed constructs was verified by using confocal Ca-imaging technologies. These FCIP-expressing virus recombinants provide a new tool for the functional analysis of whole circuits of synaptically connected neurons in vitro and in vivo.
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20105103What OpenQuestion holds
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