Evidence map›Paper›PMID 20104670›Full record

ArticleThe journal of sexual medicine2010

Treatment of erectile dysfunction in the obese type 2 diabetic ZDF rat with adipose tissue-derived stem cells.

Maurice M Garcia, Thomas M Fandel, Guiting Lin, Alan W Shindel, Lia Banie, Ching-Shwun Lin, Tom F Lue

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of sexual medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed, 4 pooled it
6.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 4 syntheses or guidelines pooled it, 147 citations in OpenAlex.

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  9. Research Advances in Stem Cell Therapy for Erectile Dysfunction.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Maurice M GarciaDepartment of Urology, University of California School of Medicine-Knuppe Molecular Urology Laboratory, San Francisco, CA 94143-0738, USA. mgarcia@urology.ucsf.edu
Thomas M Fandel
Guiting Lin
Alan W Shindel
Lia Banie
Ching-Shwun Lin
Tom F Lue
University of California, San Francisco · US

Funding

Prevention and Treatment of ImpotenceR37DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2004 to 2013
$3.5M
TREATMENT AND PREVENTION OF IMPOTENCER01DK045370 · NIDDK · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI LUE, TOM F · 1994 to 2001
$631k
NIDDK NIH HHS R01 DK045370NIDDK NIH HHS R37 DK045370
6 · The paper itself

Abstract

introductionErectile dysfunction (ED) is a major complication of type 2 diabetes, and many diabetic men with ED are refractory to common ED therapies.

aimTo determine whether autologous adipose tissue-derived stem cells (ADSCs) injected into the penis of impotent type 2 diabetic rats improve erectile function.

main outcome measuresBlood glucose levels, intracavernous pressure (ICP) increase upon cavernous nerve (CN) electrostimulation, and immunohistochemistry.

methodsTwenty-two male Zucker diabetic fatty (ZDF) rats were used. At 22 weeks of age, all the animals underwent unilateral CN electrostimulation and ICP measurement to confirm impotence. Paragonadal adipose tissue was harvested to procure ADSCs. The impotent animals were randomized to ADSC treatment and sham control groups. At 23 weeks of age, the treatment group animals underwent a penile injection of 1 million ADSCs; the control group animals received vehicle only. Erectile function studies were repeated at 26 weeks of age, followed by tissue harvest.

resultsThe rats developed diabetes within the first 10 weeks of age. At 22 weeks of age, 20 out of the 22 rats presented with ED. The post-treatment ICP increase during CN stimulation and ICP increase/mean arterial pressure were significantly higher in the treatment group compared with controls. Three weeks after injection into the corpus cavernosum, only a small number of BrdU-labeled ADSCs was detectable within corporal tissue of the treatment group. There was a significant increase in neuronal nitric oxide synthase (nNOS) in the penile dorsal nerve and in the number of endothelial cells in the corpora cavernosa of the rats in the treatment group.

conclusionAutologous ADSCs injected into the penis were effective to improve erectile function and to alter the microarchitecture of the corpus cavernosum. Since the number of ADSCs retained in the corpus cavernosum is very small, we postulate that their paracrine function, not trans-differentiation to smooth muscle or endothelial cells, is responsible for the improvement in penile function.

Indexed as

Adipose TissueAnimalsBlood GlucoseBlood PressureCell SurvivalDiabetes Mellitus, ExperimentalElectric StimulationEndothelium, VascularErectile DysfunctionImmunoenzyme TechniquesMaleMesenchymal Stem Cell TransplantationNitric Oxide SynthasePenisPeripheral NervesRatsBlood GlucoseNitric Oxide SynthaseTestosterone

Identifiers

PMID20104670
PMCPMC2904063
OpenAlexW1964837131

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.