ArticleJournal of clinical biochemistry and nutrition2010
New Strategy of Functional Analysis of PHGPx Knockout Mice Model Using Transgenic Rescue Method and Cre-LoxP System.
Article in Journal of clinical biochemistry and nutrition, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Senescent endothelial cell-derived extracellular vesicles promote neoadjuvant chemoresistance in colorectal cancer via GPX4.Communications biology · 2026Article
- Slowly progressive cell death induced by GPx4-deficiency occurs via MEK1/ERK2 activation as a downstream signal after iron-independent lipid peroxidation.Journal of clinical biochemistry and nutrition · 2024Article
- Iron Overload via Heme Degradation in the Endoplasmic Reticulum Triggers Ferroptosis in Myocardial Ischemia-Reperfusion Injury.JACC. Basic to translational science · 2022Article
- Mitochondrial regulation during male germ cell development.Cellular and molecular life sciences : CMLS · 2022Review
- Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet.Journal of clinical biochemistry and nutrition · 2021Article
- Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice.Scientific reports · 2020Article
- Mitochondria-dependent ferroptosis plays a pivotal role in doxorubicin cardiotoxicity.JCI insight · 2020Article
- Enhancement of lipid peroxidation and its amelioration by vitamin E in a subject with mutations in the SBP2 gene.Journal of lipid research · 2015Article
- Glutathione peroxidase 4 is required for maturation of photoreceptor cells.The Journal of biological chemistry · 2012Article
- Attenuation of vaccinia Tian Tan strain by removal of viral TC7L-TK2L and TA35R genes.PloS one · 2012Article
- Redox reactions in mammalian spermatogenesis and the potential targets of reactive oxygen species under oxidative stress.SpermatogenesisReview
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is an intracellular antioxidant enzyme that directly reduces peroxidized phospholipids. PHGPx is transcribed from one gene into three types of mRNA, mitochondrial, non-mitochondrial and nucleolar PHGPx by alternative transcription. In this review, we focus on our recent experiments on the regulation of promoter activity of the types of PHGPx and on the novel strategy of functional analysis of a PHGPx knockout mice model using the transgenic rescue method and Cre-LoxP system. PHGPx is especially high in testis and spermatozoa. A deficiency is implicated in human infertility. We established spermatocyte-specific PHGPx knockout (KO) mice using a Cre-loxP system. Targeted disruption of all exons of the PHGPx gene in mice by homologous recombination caused embryonic lethality at 7.5 days post coitum. The PHGPx-loxP transgene rescued PHGPx KO mice from embryonic lethality. These rescued floxed PHGPx mice were mated with spermatocyte specific Cre expressing mice. All the spermatocyte-specific PHGPx KO male mice were infertile and displayed a significant decrease in the number of spermatozoa and significant reductions in forward motility by mitochondrial dysfunction of spermatozoa. These results demonstrate that depletion of PHGPx in spermatozoa may be one of the causes of male infertility in mice and humans.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.