ArticlePhysiological genomics2010
Mammary gene expression profiles during an intramammary challenge reveal potential mechanisms linking negative energy balance with impaired immune response.
Article in Physiological genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Pooled it
- Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions.BMC veterinary research · 2024Article
- Novel lncRNA regulatory elements in milk somatic cells of Holstein dairy cows associated with mastitis.Communications biology · 2024Article
- Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis.Frontiers in cellular and infection microbiology · 2024Article
- Use of milk proteins as biomarkers of changes in the rumen metaproteome of Holstein cows fed low-fiber, high-starch diets.Journal of dairy science · 2023Article
- Candidate genes for mastitis resistance in dairy cattle: a data integration approach.Journal of animal science and biotechnology · 2023Article
- Nutrigenomic Interventions to Address Metabolic Stress and Related Disorders in Transition Cows.BioMed research international · 2022Review
- AMPK Signaling in Energy Control, Cartilage Biology, and Osteoarthritis.Frontiers in cell and developmental biology · 2021Review
- Mismatch of Glucose Allocation between Different Life Functions in the Transition Period of Dairy Cows.Animals : an open access journal from MDPI · 2020Review
- Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows.Frontiers in genetics · 2019Article
- Mucosal Immunity in the Female Murine Mammary Gland.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Whole blood transcriptome analysis reveals potential competition in metabolic pathways between negative energy balance and response to inflammatory challenge.Scientific reports · 2017Article
- Metabolic Disorders in the Transition Period Indicate that the Dairy Cows' Ability to Adapt is Overstressed.Animals : an open access journal from MDPI · 2015Review
- Sequencing the transcriptome of milk production: milk trumps mammary tissue.BMC genomics · 2013Article
- Effect of mastitis during the first lactation on production and reproduction performance of Holstein cows.Tropical animal health and production · 2012Article
- Functional adaptations of the transcriptome to mastitis-causing pathogens: the mammary gland and beyond.Journal of mammary gland biology and neoplasia · 2011Review
- Comparative expression profiling of E. coli and S. aureus inoculated primary mammary gland cells sampled from cows with different genetic predispositions for somatic cell score.Genetics, selection, evolution : GSE · 2011Article
- Predisposition of cows to mastitis in non-infected mammary glands: effects of dietary-induced negative energy balance during mid-lactation on immune-related genes.Functional & integrative genomics · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Our objective was to compare mammary tissue gene expression profiles during a Streptococcus uberis (S. uberis) mastitis challenge between lactating cows subjected to dietary-induced negative energy balance (NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5) to better understand the mechanisms associated with NEB and risk of mastitis during the transition period. The NEB cows were feed-restricted to 60% of calculated net energy for lactation requirements for 7 days, and cows assigned to PEB were fed the same diet for ad libitum intake. Five days after feed restriction, one rear mammary quarter of each cow was inoculated with 5,000 cfu of S. uberis (O140J). At 20 h postinoculation, S. uberis-infected mammary quarters from all cows were biopsied for RNA extraction. Negative energy balance resulted in 287 differentially expressed genes (DEG; false discovery rate ≤ 0.05), with 86 DEG upregulated and 201 DEG downregulated in NEB vs. PEB. Canonical pathways most affected by NEB were IL-8 signaling (10 genes), glucocorticoid receptor signaling (13), and NRF2-mediated oxidative stress response (10). Among the genes differentially expressed by NEB, cell growth and proliferation (48) and cellular development (36) were the most enriched functions. Regarding immune response, HLA-A was upregulated due to NEB, whereas the majority of genes involved in immune response were downregulated (e.g., AKT1, IRAK1, MAPK9, and TRAF6). This study provided new avenues for investigation into the mechanisms relating NEB and susceptibility to mastitis in lactating dairy cows.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.