ArticleTraffic (Copenhagen, Denmark)2010
The G-protein coupled receptor associated sorting protein GASP-1 regulates the signalling and trafficking of the viral chemokine receptor US28.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging.iScience · 2023Article
- US28, a Virally-Encoded GPCR as an Antiviral Target for Human Cytomegalovirus Infection.Biomolecules & therapeutics · 2017Review
- Gα13 mediates human cytomegalovirus-encoded chemokine receptor US28-induced cell death in melanoma.International journal of cancer · 2015Article
- Modulation of cellular signaling by herpesvirus-encoded G protein-coupled receptors.Frontiers in pharmacology · 2015Review
- Atypical regulation of G protein-coupled receptor intracellular trafficking by ubiquitination.Current opinion in cell biology · 2014Review
- Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism.Cell · 2013Article
- Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model.Journal of virology · 2013Article
- The cannabinoid receptor CB1 modulates the signaling properties of the lysophosphatidylinositol receptor GPR55.The Journal of biological chemistry · 2012Article
- The GPCR-associated sorting protein 1 regulates ligand-induced down-regulation of GPR55.British journal of pharmacology · 2012Article
- Constitutive formation of an RXFP1-signalosome: a novel paradigm in GPCR function and regulation.British journal of pharmacology · 2012Review
- P60TRP interferes with the GPCR/secretase pathway to mediate neuronal survival and synaptogenesis.Journal of cellular and molecular medicine · 2011Article
- Heteromerization of human cytomegalovirus encoded chemokine receptors.Biochemical pharmacology · 2011Article
- Role of ubiquitination in endocytic trafficking of G-protein-coupled receptors.Traffic (Copenhagen, Denmark) · 2011Review
- Dopamine D(3) receptors are down-regulated following heterologous endocytosis by a specific interaction with G protein-coupled receptor-associated sorting protein-1.The Journal of biological chemistry · 2011Article
- G protein-coupled receptor-associated sorting protein 1 regulates the postendocytic sorting of seven-transmembrane-spanning G protein-coupled receptors.Pharmacology · 2010Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
Human cytomegalovirus (HCMV) encodes the seven transmembrane(7TM)/G-protein coupled receptor (GPCR) US28, which signals and endocytoses in a constitutive, ligand-independent manner. Here we show that, following endocytosis, US28 is targeted to the lysosomes for degradation as a consequence of its interaction with the GPCR-associated sorting protein-1 (GASP-1). We find that GASP-1 binds to US28 in vitro and that disruption of the GASP-1/US28 interaction by either (i) overexpression of dominant negative cGASP-1 or by (ii) shRNA knock-down of endogenous GASP-1 is sufficient to inhibit the lysosomal targeting of US28 and slow its post-endocytic degradation. Furthermore, we found that GASP-1 affects US28-mediated signalling. The knock-down of endogenous GASP-1 impairs the US28-mediated Galphaq/PLC/inositol phosphate (IP) accumulation as well as the activation of the transcription factors Nuclear Factor-kappaB (NF-kappaB) and cyclic AMP responsive element binding protein (CREB). Overexpression of GASP-1 enhances both IP accumulation and transcription factor activity. Thus, GASP-1 is an important cellular determinant that not only regulates the post-endocytic trafficking of US28, but also regulates the signalling capacities of US28.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.