ArticleImmunology2010
Over-expression of Runx1 transcription factor impairs the development of thymocytes from the double-negative to double-positive stages.
Article in Immunology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- RUNX1 expression dynamics in plasma cell differentiation and pathogenesis of multiple myeloma.Frontiers in immunology · 2025Article
- Runx factors launch T cell and innate lymphoid programs via direct and gene network-based mechanisms.Nature immunology · 2023Article
- Epigenetic programming defines haematopoietic stem cell fate restriction.Nature cell biology · 2023Article
- Article
- Transcriptional Regulation of Early T-Lymphocyte Development in Thymus.Frontiers in immunology · 2022Review
- Dynamic control of the T-cell specification gene regulatory network.Current opinion in systems biology · 2019Article
- Transcription Factor PU.1 Represses and Activates Gene Expression in Early T Cells by Redirecting Partner Transcription Factor Binding.Immunity · 2018Article
- Apoptotic Diminution of Immature Single and Double Positive Thymocyte Subpopulations Contributes to Thymus Involution During Murine Polymicrobial Sepsis.Shock (Augusta, Ga.) · 2017Article
- Dynamic recruitment of Ets1 to both nucleosome-occupied and -depleted enhancer regions mediates a transcriptional program switch during early T-cell differentiation.Nucleic acids research · 2016Article
- An Lck-cre transgene accelerates autoantibody production and lupus development in (NZB × NZW)F1 mice.Lupus · 2016Article
- A genome wide transcriptional model of the complex response to pre-TCR signalling during thymocyte differentiation.Oncotarget · 2015Article
- Activation of HIV-1 from latent infection via synergy of RUNX1 inhibitor Ro5-3335 and SAHA.PLoS pathogens · 2014Article
- A germline point mutation in Runx1 uncouples its role in definitive hematopoiesis from differentiation.Experimental hematology · 2013Article
- The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.Molecular biology of the cell · 2013Article
- Novel function of the unique N-terminal region of RUNX1c in B cell growth regulation.Nucleic acids research · 2013Article
- Down-regulation of Runx1 expression by TCR signal involves an autoregulatory mechanism and contributes to IL-2 production.The Journal of biological chemistry · 2011Article
- Interplay of transcription factors in T-cell differentiation and function: the role of Runx.Immunology · 2011Review
- Immune Dysregulation and the Increased Risk of Complications and Mortality Following Respiratory Tract Infections in Adults With Down Syndrome.Frontiers in immunologyReview
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Runx1 transcription factor is highly expressed at a CD4/CD8-double-negative (DN) stage of thymocyte development but is down-regulated when cells proceed to the double-positive (DP) stage. In the present study, we examined whether the down-regulation of Runx1 is necessary for thymocyte differentiation from the DN to DP stage. When Runx1 was artificially over-expressed in thymocytes by Lck-driven Cre, the DN3 population was unaffected, as exemplified by proper pre-T-cell receptor expression, whereas the DN4 population was perturbed as shown by the decrease in the CD27(hi) sub-fraction. In parallel, the growth rate of DN4 cells was reduced by half, as measured by bromodeoxyuridine incorporation. These events impaired the transition of DN4 cells to the DP stage, resulting in the drastic reduction of the number of DP thymocytes. The Runx1 gene has two promoters, a proximal and a distal promoter; and, in thymocytes, endogenous Runx1 was mainly transcribed from the distal promoter. Interestingly, only distal, but not proximal, Runx1 over-expression exhibited an inhibitory effect on thymocyte differentiation, suggesting that the distal Runx1 protein may fulfil a unique function. Our collective results indicate that production of the distal Runx1 protein must be adequately down-regulated for thymocytes to transit from the DN to the DP stage, a critical step in the massive expansion of the T-cell lineage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.