ArticleContrast media & molecular imaging
Fluorescence lifetime imaging of activatable target specific molecular probes.
Article in Contrast media & molecular imaging. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Near-infrared probes for luminescence lifetime imaging.Nanotheranostics · 2022Review
- Activatable Near-Infrared Fluorescence Imaging Using PEGylated Bacteriochlorin-Based Chlorin and BODIPY-Dyads as Probes for Detecting Cancer.Bioconjugate chemistry · 2019Article
- Activatable fluorescent probes in fluorescence-guided surgery: Practical considerations.Bioorganic & medicinal chemistry · 2018Review
- Molecular probes for fluorescence lifetime imaging.Bioconjugate chemistry · 2015Review
- In vivo fluorescence lifetime imaging for monitoring the efficacy of the cancer treatment.Clinical cancer research : an official journal of the American Association for Cancer Research · 2014Article
- Multiscale BerEp4 molecular imaging of microtumor phantoms: toward theranostics for basal cell carcinoma.Molecular imaging · 2014Article
- In vivo validation of quantitative frequency domain fluorescence tomography.Journal of biomedical optics · 2012Article
- Real-time monitoring of in vivo acute necrotic cancer cell death induced by near infrared photoimmunotherapy using fluorescence lifetime imaging.Cancer research · 2012Article
- In vivo fluorescence lifetime detection of an activatable probe in infarcted myocardium.Journal of biomedical optics · 2012Article
- Recent trends in antibody-based oncologic imaging.Cancer letters · 2012Review
- In vivo fluorescence lifetime imaging monitors binding of specific probes to cancer biomarkers.PloS one · 2012Article
- Rational chemical design of the next generation of molecular imaging probes based on physics and biology: mixing modalities, colors and signals.Chemical Society reviews · 2011Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
In vivo optical imaging using fluorescently labeled self-quenched monoclonal antibodies, activated through binding and internalization within target cells, results in excellent target-to-background ratios. We hypothesized that these molecular probes could be utilized to accurately report on cellular internalization with fluorescence lifetime imaging (FLI). Two imaging probes were synthesized, consisting of the antibody trastuzumab (targeting HER2/neu) conjugated to Alexa Fluor750 in ratios of either 1:8 or 1:1. Fluorescence intensity and lifetime of each conjugate were initially determined at endosomal pHs. Since the 1:8 conjugate is self-quenched, the fluorescence lifetime of each probe was also determined after exposure to the known dequencher SDS. In vitro imaging experiments were performed using 3T3/HER2(+) and BALB/3T3 (HER2(-)) cell lines. Changes in fluorescence lifetime correlated with temperature- and time-dependent cellular internalization. In vivo imaging studies in mice with dual flank tumors [3T3/HER2(+) and BALB/3T3 (HER2(-))] detected a minimal difference in FLI. In conclusion, fluorescence lifetime imaging monitors the internalization of target-specific activatable antibody-fluorophore conjugates in vitro. Challenges remain in adapting this methodology to in vivo imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.