ArticleNature methods2010
Systems analysis of EGF receptor signaling dynamics with microwestern arrays.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
What it found
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Who cites it
112 citing papers in PubMed.
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- Label-Free Single-Molecule Pulldown for the Detection of Released Cellular Protein Complexes.ACS central science · 2022Article
- Application of Micro-Western Array for Identifying Different Serum Protein Expression Profile among Healthy Control, Alzheimer's Disease Patients and Patients' Adult Children.Brain sciences · 2022Article
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- Practical application of a Bayesian network approach to poultry epigenetics and stress.BMC bioinformatics · 2022Article
- Screening of organoids derived from patients with breast cancer implicates the repressor NCOR2 in cytotoxic stress response and antitumor immunity.Nature cancer · 2022Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Disruption of the pentraxin 3/CD44 interaction as an efficient therapy for triple-negative breast cancers.Clinical and translational medicine · 2022Article
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- Novel Virulence Role of Pneumococcal NanA in Host Inflammation and Cell Death Through the Activation of Inflammasome and the Caspase Pathway.Frontiers in cellular and infection microbiology · 2021Article
- Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.Nature biomedical engineering · 2021Article
- Computational Phosphorylation Network Reconstruction: An Update on Methods and Resources.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Investigating the Evolution and Development of Biological Systems from the Perspective of Thermo-Kinetics and Systems Theory.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2020Review
- Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology.Journal of visualized experiments : JoVE · 2020Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe microwestern arrays, which enable quantitative, sensitive and high-throughput assessment of protein abundance and modifications after electrophoretic separation of microarrayed cell lysates. This method allowed us to measure 91 phosphosites on 67 proteins at six time points after stimulation with five epidermal growth factor (EGF) concentrations in A431 human carcinoma cells. We inferred the connectivities among 15 phosphorylation sites in 10 receptor tyrosine kinases (RTKs) and two sites from Src kinase using Bayesian network modeling and two mutual information-based methods; the three inference methods yielded substantial agreement on the network topology. These results imply multiple distinct RTK coactivation mechanisms and support the notion that small amounts of experimental data collected from phenotypically diverse network states may enable network inference.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.