Evidence map›Paper›PMID 20101096›Full record

ArticleJournal of endocrinological investigation

Suppression effects of AICAR on insulin secretion involved in peroxisome proliferator-activated receptor gamma changes in INS-1 cells.

H Guo, X J Zhang, F Wang, Y Wang, Y Shen, J J Zhao, L Gao

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In one paragraph

Article in Journal of endocrinological investigation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

H GuoCentral Laboratory, Provincial Hospital affiliated to Shandong University, No. 324, Jing 5 Road, Jinan, 250021, China. gaoling1@medmail.com.cn
X J Zhang
F Wang
Y Wang
Y Shen
J J Zhao
L Gao
Shandong Provincial Hospital · CNShandong University · CNJinan Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAMP-activated protein kinase (AMPK) activation is known to attenuate glucose-stimulated insulin secretion (GSIS) in pancreatic beta cells. However, the underlying mechanisms are poorly understood. The purpose of this study was to examine the effects of AMPK activation on insulin secretion and to determine whether peroxisome proliferator-activated receptors (PPAR) are involved in the effects on INS-1 cells.

methodsINS-1 cells, insulinoma cell lines, were treated with an activator (AICAR) or inhibitor (Compound C) of AMPK as well as inhibitors of PPAR [MK886 and biphenol A diglycidyl ether (BADGE)] for different treatment times.

resultsAICAR-induced AMPK activation significantly attenuated GSIS as well as insulin content. Meanwhile, AMPK activation increased the mRNA levels of both PPARalpha and PPARgamma. However, with regard to DNA binding, AMPK activation upregulated PPARgamma only, and it was possible to reduce the increment with the AMPK inhibitor. Moreover, the AICAR-induced suppression of insulin secretion can be counteracted by the PPARgamma inhibitor, BADGE but not the PPARalpha inhibitor.

conclusionsAICAR-induced glucose-stimulated insulin secretion reduction correlates mainly with PPARgamma changes.

Indexed as

Aminoimidazole CarboxamideAMP-Activated Protein KinasesAnimalsBenzhydryl CompoundsBisphenol A CompoundsCell Line, TumorChlorobenzenesEnzyme ActivationEpoxy CompoundsGlucoseIndolesInsulinInsulinomaInsulin SecretionPPAR alphaPPAR gammaAICA ribonucleotideAminoimidazole CarboxamideAMP-Activated Protein KinasesBenzhydryl CompoundsBisphenol A Compoundsbisphenol A diglycidyl etherChlorobenzenesdorsomorphinEpoxy CompoundsGlucoseIndolesInsulinMK-886PPAR alphaPPAR gammaPyrazolesPyrimidinesRibonucleotides

Identifiers

PMID20101096
OpenAlexW1978134116

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.